Recombinant Human NEDD8 Agarose Protein, CF

Product Discontinued

Recombinant Human NEDD8 Agarose Protein, CF Summary

• Details of Functionality: NEDD8 Agarose is ideal for the enrichment of known NEDD8-interacting proteins as well as the discovery of novel NEDD8-interacting proteins. We recommend equilibrating the resin by washing with 5-10 mL of your desired aqueous buffer.
• Source: E. coli-derived human NEDD8 protein
• Accession #: Q15843
• Protein/Peptide Type: Recombinant Proteins
• Gene: NEDD8

Applications/Dilutions

• Dilutions:
  • Enzyme Activity

Packaging, Storage & Formulations

• Storage: Do not freeze.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
• Buffer: 1 ml total volume of Hepes buffered solution.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NEDD8 Agarose Protein, CF

• FLJ43224
• MGC104393
• MGC125896
• MGC125897
• NEDD8
• NEDD-8
• Neddylin
• Neural precursor cell expressed developmentally down-regulated protein 8
• neural precursor cell expressed, developmentally down-regulated 8
• Rub1
• Ubiquitin-like protein Nedd8

Background

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393.
2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.

Bioinformatics

• Gene Symbol: NEDD8
• Uniprot:
  • Q15843(Human)