Recombinant Human MICA Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human MICA Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind rhNKG2D/Fc Chimera in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived human MICA protein
  

  Human MICA (Ala23 - Gln308) Accession # NP_001170990	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_001170990
• N-terminal Sequence: Ala23
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: MICA
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 59 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MICA Fc Chimera Protein, CF

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. NKG2D and its Ligands (2002) http://www.RnDSystems.com

Publications for MICA (1300-MA)(12)

Publications using 1300-MA

• Vu, LQ;Espinoza, JL;Nguyen, HTG;Mizuno, S;Takami, A; MAIT Cells in the Bone Marrow of Patients with Aplastic Anemia International journal of molecular sciences 2024-09-21 [PMID: 39337644] (Bioassay, Human)
• Seller, A;Tegeler, CM;Mauermann, J;Schreiber, T;Hagelstein, I;Liebel, K;Koch, A;Heitmann, JS;Greiner, SM;Hayn, C;Dannehl, D;Engler, T;Hartkopf, AD;Hahn, M;Brucker, SY;Salih, HR;Märklin, M; Soluble NKG2DLs Are Elevated in Breast Cancer Patients and Associate with Disease Outcome International journal of molecular sciences 2024-04-08 [PMID: 38612935] (ELISA Standard, Human)
• JA Myers, D Schirm, L Bendzick, R Hopps, C Selleck, P Hinderlie, M Felices, JS Miller Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion JCI Insight, 2022-08-08;0(0):. 2022-08-08 [PMID: 35727627] (Bioassay, Human)
• A Gil-Krzews, MB Saeed, A Oszmiana, ER Fischer, K Lagrue, WA Gahl, WJ Introne, JE Coligan, DM Davis, K Krzewski An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome J. Allergy Clin. Immunol., 2017-12-11;0(0):. 2017-12-11 [PMID: 29241728] (Bioassay, Human)
• AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017-05-09;8(19):31959-31976. 2017-05-09 [PMID: 28404876] (ELISA (Standard), Human)
• Lagrue K, Carisey A, Morgan D, Chopra R, Davis D Lenalidomide augments actin remodeling and lowers NK-cell activation thresholds. Blood, 2015-05-22;126(1):50-60. 2015-05-22 [PMID: 26002964] (Bioassay, Human)
• Brown A, Dobbie I, Alakoskela J, Davis I, Davis D Super-resolution imaging of remodeled synaptic actin reveals different synergies between NK cell receptors and integrins. Blood, 2012-09-10;120(18):3729-40. 2012-09-10 [PMID: 22966166] (Bioassay, Human)
• Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS. J. Virol., 2010-06-09;84(16):8141-52. 2010-06-09 [PMID: 20534857] (ELISA (Standard))
• Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia. Leukemia, 2010-04-29;24(6):1152-9. 2010-04-29 [PMID: 20428196] (ELISA (Standard))
• Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J. Mol. Biol., 2008-09-16;384(5):1143-56. 2008-09-16 [PMID: 18809410] (Bioassay, Human)
• Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities. Blood, 2007-11-09;111(3):1428-36. 2007-11-09 [PMID: 17993609] (ELISA (Standard))
• Spreu J, Stehle T, Steinle A Human cytomegalovirus-encoded UL16 discriminates MIC molecules by their alpha2 domains. J. Immunol., 2006-09-01;177(5):3143-9. 2006-09-01 [PMID: 16920952] (Binding Assay, Human)

Bioinformatics

• Gene Symbol: MICA
• Uniprot:
  • NP_001170990()