Recombinant Human mGluR7 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human mGluR7 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human mGluR7 protein
  Arg33 & Gln35-Ser521, with a C-terminal 6-His tag
• Accession #: Q14831-1
• N-terminal Sequence: Arg33; Gln35 inferred from enzymatic pyroglutamate treatment revealing Glu36
• Structure / Form:

  Disulfide-linked homodimer

• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-68 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human mGluR7 Protein, CF

• FLJ40498
• glutamate receptor, metabotropic 7
• GPRC1G
• GRM7
• metabotropic glutamate receptor 7
• MGLU7
• mGluR7
• MGLUR7GPRC1GGLUR7

Background

Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domains, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulated by phosphorylation, and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). Mature human mGluR8 is 875 amino acids in length, including a 550 amino acid (aa) N-terminal ECD (4). Within N-terminal ECD, human mGluR8 shares 98.4% and 98.5% aa sequence identity with mouse and rat mGluR8, respectively.

1. Conn P.J. and J.P. Pin (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
2. Niswender C.M. and P.J. Conn (2010) Annu. Rev. Pharmacol. Toxicol. 50:295.
3. Pin J.P. and Duvoisin R. (1995) Neuropharmacology. 34:1.
4. Makoff A. et al. (1996) Brain Res. Mol. Brain. Res. 40:165.

Bioinformatics

• Uniprot:
  • Q14831-1()