Recombinant Human MDGA2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MDGA2 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 7.5-30 μg on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human MDGA2 protein
  Gln21-Asp931, with a C-terminal 6-His tag
• Accession #: Q7Z553
• N-terminal Sequence: No results obtained: Gln21 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: MDGA2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 103.2 kDa .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-125 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MDGA2 Protein, CF

• MAM domain containing glycosylphosphatidylinositol anchor 2
• MAMDC1
• MDGA2

Background

MDGA2 (MAM domain-containing glycosylphosphatidylinositol anchor protein 2; also named MAMDC1) is a 130 kDa member of the Ig superfamily of proteins (1). Human MDGA2 is synthesized as a 956 amino acid (aa) precursor that contains a 25 aa signal sequence, a 906 aa mature chain, and a 25 aa propeptide. The mature chain consists of six Ig-like domains, followed by a MAM domain (aa 746 - 921) and a GPI anchor. In addition, there are eight potential sites for N-linked glycosylation. Mature human MDGA2 shares 98% aa sequence identity with mature mouse and rat MDGA2. MDGA2 is structurally similar to other IgCAMS, such as the L1 family and axonin 1, which have roles in cell adhesion, migration, and process outgrowth (2). Northern blot analysis shows MDGA2 expression is limited to the central and peripheral nervous system (1). Within the brain, moderate expression is observed in the cerebral cortex, the hindbrain, the basilar pons, the neocortex, the hippocampus, the amygdala, olfactory bulb, and selected nuclei of the thalamus (1). The similarity of MDGA2 to other Ig-containing molecules, and its temporal-spatial patterns of expression within restricted neuronal populations, suggest a role for MDGA2 in regulating neuronal migration, as well as other aspects of neural development, including axon guidance (1). One study shows that MDGA2 gene is implicated in neuroticism (3).

1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
2. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
3. van den Oord, E.J. et al. (2008) Arch Gen Psychiatry 65:1062. 

Bioinformatics

• Gene Symbol: MDGA2
• Uniprot:
  • Q7Z553(Human)