Recombinant Human LTK Protein, CF

When Recombinant Human LTK (Catalog # 9376-LK) is immobilizedat 2 μg/mL, 100 μL/well, Recombinant Human FAM-150A binds with an ED50 of 40-240 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LTK Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human LTK is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human FAM-150A that produces 50% of the optimal binding response is 40-240 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human LTK protein
  Ser20-Pro424, with a C-terminal His-tag
• Accession #: P29376-1
• N-terminal Sequence: Ser20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 56-84 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LTK Protein, CF

• EC 2.7.10.1
• leukocyte receptor tyrosine kinase
• leukocyte tyrosine kinase receptor
• leukocyte tyrosine kinase
• LTK
• TYK1
• TYK1Protein tyrosine kinase 1

Background

LTK, also known as Leukocyte tyrosine kinase receptor and protein tyrosine kinase 1, is a type I transmembrane protein and shares a high degree of sequence similarity with kinases in the insulin receptor family (1). Alternative splicing generates several isoforms of 50-100 kDa (2). LTK contains a 408 aa extracellular domain, 25 aa transmembrane domain, and 415 aa cytoplasmic domain. The cytoplasmic region has several phosphotyrosines that interact with downstream signaling molecules (3). LTK is reported to undergo dimerand trimerization when under certain conditions. The extracellular domain of human LTK shares 75% homology with mouse LTK. It is expressed in preB lymphocytes, B lymphocytes, and other hematopoietic cells, as well as brain and placenta (48). Human LTK can bind to PLCgamma 1, PI3K, GAP and Raf1 in vivo (6). LTK promotes neurite outgrowth via PI3K/AKT and Ras/MAPK pathways (7). FAM150A and FAM150B can both bind to the extracellular domain of LTK, but only FAM150A is the specific ligand for LTK (9, 10).

1. Honda, H. et al. (1999) Oncogene 18:3821.
2. Yazaki, Y. et al. (1993) Proc Natl. Acad. Sci. U.S.A. 90:5404.
3. Maru, Y., et al. (1990) Oncogene Res. 5:19.
4. Ben-Neriah, Y. et al. (1988) Nature 333:672.
5. Bernards, A. et al. (1990) EMBO J. 9:2279.
6. Kozutsumi, H et al. (1994) Oncogene 9:2991.
7. Yamada, S. et al. (2008) Neuroreport 19:1733.
8. Roll, JD. et al. (2012) PLoS One 7:e31733.
9. Reshetnyak, AV. et al. (2015) Proc Natl Acad Sci U S A. 112:15862.
10. Guan, J, AV. et al. (2015) Elife. 4:e09811.

Bioinformatics

• Uniprot:
  • P29376-1()