Recombinant Human Latent TGF-beta bp4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Latent TGF-beta bp4 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Latent TGF-beta bp4 protein
Met1-Ala1624, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Multiple starts between Met1 & Ser273
Protein/Peptide Type
Recombinant Proteins
Gene
LTBP4
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
174 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
155-205 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Latent TGF-beta bp4 Protein, CF

  • Latent TGFbeta bp4
  • Latent TGF-beta bp4
  • latent transforming growth factor beta binding protein 4
  • LTBP4
  • LTBP-4
  • LTBP4L
  • LTBP4S

Background

Latent TGF‑ beta Binding Protein‑4 (LTBP‑4) is a widely expressed 170‑230 kDa secreted glycoprotein within the LTBP/fibrillin family (1‑3). LTBPs contain a conserved pattern of eight-cysteine motifs (8Cys) and EGF‑like (EGFL) domains (2‑4). The 1624 amino acid (aa) human LTBP‑4 (L isoform) includes a signal sequence (aa 1‑27), a heparin-binding N‑terminal domain (aa 28‑460) with two EGFL, one hybrid and one 8‑Cys motif, a protease-sensitive hinge region, a central region with 10 EGFL motifs, and a C‑terminal domain (aa 1180‑1624) with two EGFL and two 8Cys (1‑4). The first C‑terminal 8Cys motif is responsible for covalent association with the latency associated peptide (LAP) of latent TGF‑ beta 1 (1‑4). Unlike LTBP-1 and ‑3, LTBP‑4 does not bind TGF‑ beta 2 or TGF‑ beta 3 LAPs (4). LTBP‑4 also binds fibronectin, which mediates targeting to extracellular matrix (ECM), fibrillin-1 which mediates binding to microfibrils, and the cytokine midkine (1, 5, 6). Mature human LTBP‑4 (LTBP‑4L) shares 87% and 90% aa sequence identity with mouse and rat LTBP‑4, respectively. A variant that lacks the TGF‑binding 8Cys motif is reported to be widely expressed along with LTBP‑4L (3). Other differentially expressed human splice isoforms of 1557 (LTBP‑4S), 1587 and 636 aa have alternate N‑termini through aa 69, 151, and 919 (also lacking aa 1005‑1090), respectively (1, 7). LTBP‑4 is involved in the assembly, secretion and targeting of latent TGF‑ beta 1 to sites where it can be activated. Deletion or mutation in humans can cause Urban‑Rifkin‑Davis syndrome (URDS), a syndrome of impaired pulmonary, gastrointestinal, genitourinary, musculoskeletal and dermal development mediated by enhanced TGF‑ beta 1 activity (8). Similar defects are found in mice deleted for LTBP‑4 (9‑11). Impaired LTBP‑4 expression enhances susceptibility to colon cancer (8). LTBP-4 is also down‑regulated in breast cancer tissues (5).

  1. Kantola, A.K. et al. (2008) Exp. Cell Res. 314:2488.
  2. Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.
  3. Koli, K. et al. (2001) J. Cell Sci. 114:2869.
  4. Saharinen, J. and J. Keski-Oja (2000) Mol. Biol. Cell 11:2691.
  5. Takahashi, Y. et al. (2012) Breast Cancer 19:46.
  6. Yan, Q. et al. (2012) Hepatobiliary Pancreat. Dis. Int. 11:272.
  7. Kantola, A.K. et al. (2010) J. Cell Physiol. 223:727.
  8. Urban, Z. et al. (2009) Am. J. Hum. Genet. 85:593.
  9. Sterner-Kock, A. et al. (2002) Genes Dev. 16:2264.
  10. Dabovic, B. et al. (2009) J. Cell Physiol. 219:14.
  11. Koli, K. et al. (2004) J. Cell Biol. 167:123.

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Bioinformatics

Gene Symbol LTBP4
Uniprot