Recombinant Human Kynureninase Protein, CF

Images

 
There are currently no images for Kynureninase (4887-KH).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Kynureninase Protein, CF Summary

Details of Functionality
Measured by its ability to oxidize 3-hydroxy kynurenine. The specific activity is > 75 pmol/min/µg, as measured under the described conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Kynureninase protein
Met1-Asn465, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Met1
Protein/Peptide Type
Recombinant Enzymes
Gene
KYNU
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
51 kDa, reducing conditions
Publications
Read Publication using
4887-KH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in MES and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 50 mM Tris, 0.05% (w/v) Brij-35, 5 µM Pyridoxal Phosphate (Sigma, Catalog # P9255), pH 8.0
  • Recombinant Human Kynureninase (rhKYNU) (Catalog # 4887-KH)
  • Substrate: 3-hydroxy-DL-kynurenine (3-HK) (Sigma, Cat. # H1771), 10 mM in 50% DMSO, 50% deionized water
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhKYNU to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load in a black well plate 50 µL of 2 ng/µL rhKYNU, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 μL Assay Buffer and 50 µL 200 µM Substrate.
  4. Read at excitation and emission wavelengths of 315 nm and 415 nm (top read), respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 3-hydroxyanthranilinic acid (Sigma, Catalog # H9391).

Per Well:
  • rhKYNU: 0.1 µg
  • Substrate: 100 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Kynureninase Protein, CF

  • EC 3.7.1.3
  • KYNU
  • kynureninase (L-kynurenine hydrolase)
  • Kynureninase
  • L-kynurenine hydrolase

Background

Kynureninase is a pyridoxal-5 (-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3-hydroxyanthranilic acid and alanine (1). The enzyme is a member of the “kynurenine pathway” enzymes, through which the majority of dietary tryptophan is degraded in the liver, and is involved in the de novo biosynthesis of NAD+ (2, 3). Kynurenine pathway genes are expressed in immune system cells such as macrophages and microglia. During inflammatory responses, the kynurenine pathway in these cells produces quinolinic acid (QA) and not NAD+. QA excites neurons via the activation of NMDA (N-methyl-D-aspartate) receptors resulting in neuronal damage. The tissue-damaging process has been demonstrated in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease. Because Kynureninase is one of the key enzymes of QA production, its inhibitors may be useful for the treatment of neurological disorders. The recombinant Kynureninase has been shown to possess specificity for 3-hydroxykynurenine over kynurenine (4, 5).

  1. Lima, S. et al. (2007) Biochemistry 46:2735.
  2. Botting, N. P. (1995) Chem. Soc. Rev. 24:401.
  3. Stone, T. W. (2000) Trends in Pharm. Sci. 21:149.
  4. Walsh, H. et al. (2002) Eur. J. Chem. 269:2069.
  5. Toma, S. et al. (1997) FEBS Lett. 408:5.

Customers Who Viewed This Item Also Viewed...

H00006999-B01P
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
8050-KM
Species: Hu
Applications: EnzAct
NBP1-85892
Species: Hu
Applications: IHC, IHC-P
NBP1-87702
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-24646
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: IHC, IHC-P, WB
AF5724
Species: Hu, Mu, Rt
Applications: ICC, WB
NBP2-27461
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP2-94646
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-87781
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-89627
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-86108
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-42388
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
7954-GM/CF
Species: Hu
Applications: BA
H00005997-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, KD, WB
NBP2-94140
Species: Hu, Mu, Rt
Applications: WB
NBP1-91654
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
4887-KH
Species: Hu
Applications: Enzyme Activity

Publications for Kynureninase (4887-KH)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
N/A
(1)
All Species

Reviews for Kynureninase (4887-KH) (0)

There are no reviews for Kynureninase (4887-KH). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Kynureninase (4887-KH) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Kynureninase Products

Bioinformatics Tool for Kynureninase (4887-KH)

Discover related pathways, diseases and genes to Kynureninase (4887-KH). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Kynureninase (4887-KH)

Discover more about diseases related to Kynureninase (4887-KH).
 

Pathways for Kynureninase (4887-KH)

View related products by pathway.

PTMs for Kynureninase (4887-KH)

Learn more about PTMs related to Kynureninase (4887-KH).

Blogs on Kynureninase

There are no specific blogs for Kynureninase, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Kynureninase Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol KYNU
Uniprot