Recombinant Human Integrin alpha E beta 7 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Integrin alpha E beta 7 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When rhE-Cadherin is coated at 2 μg/mL, rhIntegrin alpha E beta 7 binds with an apparent K_d <5 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha E beta 7 protein
  

  Human Integrin alpha E (Phe19-Ser1124 (Ile477Val & Arg482Gln)) Accession # P38570	GGGSGGGS	Acidic Tail	6-His tag
  Human Integrin beta 7 (Glu20-His723) Accession # P26010	His-Pro	GGGSGGGS	Basic Tail
  N-terminus			C-terminus

• Accession #: P38570 (Integrin alpha E) & P26010 (Integrin beta 7)
• N-terminal Sequence: Phe19 ( alpha E) & Glu20 ( beta 7)
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 130.7 kDa ( alpha E) & 84.7 kDa ( beta 7).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150-170 kDa & 120-130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha E beta 7 Protein, CF

• Integrin alpha E beta 7

Background

Integrin alpha E beta 7, also called HML-1 (human mucosal lymphocyte antigen), is the only known integrin family adhesion receptor containing the alpha E (epithelial-associated) subunit, and shares the beta 7 subunit with alpha 4 beta 7 (1-3). alpha E beta 7 is the non‑covalent multimer of alpha E (designated CD103), present as 150 kDa and 25 kDa heavy and light chains, and 130 kDa beta 7 type I transmembrane glycoprotein subunits (2, 3). The alpha E extracellular N-terminal beta -propeller structure contains X (which contains the cleavage site) and I domains, and is followed by domains called thigh, calf-1 and calf-2 (1, 2). The beta 7 ECD contains a vWFA domain, which interacts with the alpha E beta ‑propeller to form a binding domain. The MIDAS motif (metal ion‑dependent adhesion site) is critical for binding of alpha E beta 7 to its ligand (4). Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 1105 aa human alpha E extracellular domain shares 70-79% aa sequence identity with mouse, rat, equine, bovine, canine and feline alpha E, while the 705 aa human beta 7 ECD shares 87%, 87%, 91% and 88% aa identity with mouse, rat, equine and bovine beta 7, respectively. alpha E beta 7 is mainly restricted to mucosal tissues, where it engages the epithelial adhesion molecule E-cadherin (4-6). It was first identified as a marker of intestinal intra-epithelial lymphocytes (2, 5, 6). It has since been recognized that a variety of leukocytes, such as cytotoxic CD8⁺ T cells, some dendritic cells, and effector/memory-like regulatory T cells, acquire alpha E beta 7 in the days following their migration to epithelial tissues such as the intestines, lungs, and epithelial layers of tonsils (6-13). In these tissues alpha E beta 7 facilitates immune surveillance, including destruction of infected or transformed epithelial cells and induction of T cell adaptive responses (7-13). Pathologically, alpha E beta 7 may be involved in allograft rejection of transplanted pancreatic islets and other tissues (14).

1. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
2. Shaw, S.K. et al. (1994) J. Biol. Chem. 269:6016.
3. Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.
4. Higgins, J.M.G. et al. (2000) J. Biol. Chem. 275:25652.
5. Cepek, K.L. et al. (1994) Nature 372:190.
6. Wagner, N. et al. (1996) Nature 382:366.
7. Le Floc’h, A. et al. (2007) J. Exp. Med. 204:559.
8. Smyth, L.J.C. et al. (2007) Clin. Exp. Immunol. 149:162.
9. Woodberry, T. et al. (2005) J. Immunol. 175:4355.
10. Jaensson, E. et al. (2008) J. Exp. Med. 205:2139.
11. del Rio, M.-L. et al. (2008) J. Immunol. 181:6178.
12. Sung, S.J. et al. (2006) J. Immunol. 176:2161.
13. Siewert, C. et al. (2008) J. Immunol. 180:146.
14. Feng, Y. et al. (2002) J. Exp. Med. 196:877.

Bioinformatics

• Uniprot:
  • P38570 (Integrin alpha E) & P26010 (Integrin beta 7)()