Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Mouse Laminin I (Catalog # 3400-010-02) is coated at 10 μg/mL, Recombinant Human Integrin alpha 6 beta 4 binds with an apparent KD <5 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 6 beta 4 protein
Human Integrin alpha 6
(Phe24-Lys878)
Accession # NP_000201
Acidic Tail HHHHHH
Human Integrin beta 4
(Asn28-Ser710)
Accession # P16144
Basic Tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe24 ( alpha 6) & Asn28 ( beta 4)
Protein/Peptide Type
Recombinant Proteins
Gene
ITGA6
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
104 kDa ( alpha 6) & 84.8 kDa ( beta 4).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
125-135 kDa ( alpha 6) & 100-110 kDa ( beta 4), reducing conditions
Publications
Read Publications using
5497-A6 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 6 (X1) beta 4 Protein, CF

  • Integrin alpha 6 beta 4

Background

Integrin alpha 6 beta 4 is an epithelial and Schwann cell laminin-binding integrin. While alpha 6 can also pair with beta 1, beta 4 pairs only with alpha 6 (1-3). Expression of the non-covalent heterodimer composed of a ~150 kDa alpha 6/CD49f and 150-200 kDa beta 4/CD104 type I transmembrane glycoprotein subunit is required for hemidesmosome formation (2, 4). The alpha 6 subunit contains an I (inhibitory) domain and a cleavage site that creates extracellular heavy and transmembrane light chains. Alternative splicing in the alpha 6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a cytoplasmic site creates A and B isoforms (5, 6). The cytoplasmic domain of beta 4 is unusually long (~1000 aa) and contains four type III fibronectin repeats that bind intracellular hemidesmosomal components (2, 4, 7). Alternative splicing between repeats # 2 and 3 creates A, B, C and (truncated) D isoforms (7). All ECD variants share similar ligand binding characteristics, while cytoplasmic variants differ in tissue distribution and signaling pathways (5-7). The 683 aa human alpha 6X1 heavy chain shares 94-95% aa identity with mouse, rat, bovine, and canine a6, and the 683 aa human beta 4 ECD shares 87-92% aa identity with mouse, rat, bovine, and equine beta 4. Mutation of alpha 6 beta 4 can cause EB-PA, or epidermolysis bullosa (detachment of epidermis from basement membrane) with pyloric atresia, that is neonatally lethal if severe (8). On Schwann cells, alpha 6 beta 4 cooperates with dystroglycan to stabilize the myelin sheath, and mediates attachment to the basal lamina (9, 10). High alpha 6 beta 4 expression correlates with invasiveness of carcinomas (2). EGF R-induced phosphorylation of beta 4 disrupts hemidesmosomes and allows tumor cell migration (11, 12). alpha 6 beta 4 signaling can also amplify tumor production of VEGF and ErbB proteins (13, 14).
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Wilhelmsen, K. et al. (2006) Mol. Cell. Biol. 26:2877.
  3. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
  4. Schaapveld, R. Q. J. et al. (1998) J. Cell Biol. 142:271.
  5. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
  6. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
  7. van Leusden, M. R. et al. (1997) Biochem. Biophys. Res. Commun. 235:826.
  8. Pulkkinen, L. et al. (1998) Am. J. Hum. Genet. 63:1376.
  9. Nodari, A. et al. (2008) J. Neurosci. 28:6714.
  10. Amici, S. A. et al. (2006) J. Neurosci. 26:1179.
  11. Wilhelmsen, K. et al. (2007) Mol. Biol. Cell 18:3512.
  12. Yang, X. H. et al. (2008) Cancer Res. 68:3204.
  13. Lipscomb, E. A. et al. (2005) Cancer Res. 65:10970.
  14. Folgiero, V. et al. (2007) Cancer Res. 67:1645.

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Publications for Integrin alpha 6 beta 4 (5497-A6)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), Enzyme Assay, Surface Plasmon Resonance.


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Bioassay
(1)
ELISA (Standard)
(1)
Enzyme Assay
(1)
Surface Plasmon Resonance
(1)
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(2)
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