Recombinant Human IL1RAPL1 His-tag Protein, CF

When Recombinant Human IL1RAPL1 His-tag Protein (Catalog # 11080-MR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (9995-PR) binds with an ED50 of 6.00-60.0 ng/mL.

2 μg/lane of Recombinant Human IL1RAPL1 His-tag Protein (Catalog # 11080-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-59 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL1RAPL1 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL1RAPL1 His-tag (Catalog # 11080-MR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED₅₀ of 6.00-60.0 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IL1RAPL1 protein
  Leu19-Thr357, with a C-terminal 6-His tag
• Accession #: NP_055086.1
• N-terminal Sequence: Leu19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42-59 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL1RAPL1 His-tag Protein, CF

• IL1R8IL1RAPL
• IL-1R9
• IL1RAPL1
• IL-1RAPL1
• IL-1-RAPL-1
• IL-1RAPL-1
• IL1RAPL-1
• interleukin 1 receptor accessory protein-like 1
• interleukin 1 receptor-8
• interleukin-1 receptor accessory protein-like 1
• mental retardation, X-linked 10
• MRX10
• MRX21
• MRX34
• oligophrenin-4
• OPHN4mental retardation, X-linked 21
• Three immunoglobulin domain-containing IL-1 receptor-related 2
• TIGIRR-2
• TIGIRR-2mental retardation, X-linked 34
• X-linked interleukin-1 receptor accessory protein-like 1

Background

Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), also known as Oligophenin-4 (OPHN4) and three immunoglobulin domain containing IL-1 receptor-related 2 (TIGIRR-2) (1), is a member of the IL-1 receptor superfamily. IL1RAPL1 is a single pass type I membrane protein which contains an N-terminal signal peptide (aa 1-18 ), three extracellular immunoglobulin-like domains (aa 19-350), a transmembrane domain (aa 358-378 ), an intracellular Toll/IL-1R domain (aa 403-562), and a long C-terminal tail which interacts with multiple signaling molecules (aa 549-644 ) (2). High expression levels of IL1RAPL1 was found in post-natal hippocampus, and its expression is upregulated by neuronal activity (3). The extracellular domain of IL1RAPL1 can mediate synapse formation through trans-synaptic interaction with PTPRD (4, 5). In neurons, IL1RAPL1 interacts with PSD-95, a major scaffolding protein of excitatory synapses, and modulates its synaptic localization by regulating JNK activity and PSD-95 phosphorylation (3).  Mutation or deletion of IL1RAPL1 gene is associated with non-syndromic intellectual disability and autism spectrum disorder (5). Human IL1RAPL1 shares 98% and 99% aa sequence identity with mouse and rat IL1RAPL1, respectively.

1. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
2. Bahi, N. et al. (2003) Hum. Mol. Gen. 12:1415.
3. Pavlowsky, A. et al. (2010) Curr. Biol. 20:103.
4. Yoshida, T. et al. (2011) J. Neurosci. 31:13485.
5. Ramos-Brossier, M. et al. (2015) Hum Mol Genet. 24:1106.

Bioinformatics

• Uniprot:
  • NP_055086.1(Human)