Recombinant Human IL-9R (NS0-expressed) Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-9R (NS0-expressed) Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the IL-9-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 1-4 µg/mL in the presence of 2 ng/mL of recombinant human IL-9.
• Source: Mouse myeloma cell line, NS0-derived human IL-9 R protein
  Val40-Pro270, with a C-terminal 10-His tag
• Accession #: Q01113
• N-terminal Sequence: Val40
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL9R
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 27.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-9R (NS0-expressed) Protein, CF

• CD129 antigen
• CD129
• IL-9 R
• IL-9 receptor
• IL9R
• IL-9R
• interleukin 9 receptor
• interleukin-9 receptor

Background

The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha ) subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. The cDNAs encoding mouse and human IL-9 receptors have been isolated. The deduced mouse and human transmembrane proteins, sharing 53% amino acid sequence identity, contain 468 and 533 amino acid residues, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.

1. Renauld, J. et al. (1992) Proc. Natl. Acad. Sci. USA, 89:5690.
2. Kimura, Y. et al. (1995) Int. Immunol. 7:115.

Publications for IL-9R (290-RNS/CF)(1)

Publication using 290-RNS/CF

• KE Harris, KJ Lorentsen, HK Malik-Chau, K Loughlin, HM Basappa, S Hartstein, G Ahmil, NS Allen, BC Avanzino, A Balasubram, AA Boudreau, K Chang, MC Cuturi, LM Davison, DM Ho, S Iyer, US Rangaswamy, P Sankaran, U Schellenbe, R Buelow, ND Trinklein A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cells Scientific Reports, 2021-05-19;11(1):10592. 2021-05-19 [PMID: 34011961] (Bioassay, N/A)

Bioinformatics

• Gene Symbol: IL9R
• Uniprot:
  • Q01113()