Recombinant Human IL-31RA Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IL-31RA Protein, CF Summary

• Details of Functionality: Measured by its ability to bind rhIL-31 in a functional ELISA with an estimated K_D < 4 nM.
• Source: Mouse myeloma cell line, NS0-derived human IL-31 RA protein
  Ala20-Ser516, with a C-terminal 6-His tag
• Accession #: Q8NI17
• N-terminal Sequence: Ala20
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL31RA
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 57.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-31RA Protein, CF

• class I cytokine receptor
• CRL3
• CRL3Glmr
• Cytokine receptor-like 3
• GLMR
• GLM-R
• GLM-RMGC125346
• GP130 like receptor
• Gp130-like monocyte receptor
• Gp130-like receptor
• hGLM-R
• IL-31 RA
• IL-31 receptor subunit alpha
• IL-31R subunit alpha
• IL31RA
• IL-31RA
• IL-31RAGPLCRL
• IL-31R-alpha
• interleukin 31 receptor A
• interleukin-31 receptor subunit alpha
• PRO21384
• soluble type I cytokine receptor CRL3
• zcytoR17

Background

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co-expression with OSM-R determines a cell’s response to IL-31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).

1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
6. Bando, T. et al. (2006) Neuroscience 142:1263.
7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
8. Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
9. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
10. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.

Publications for IL-31RA (2769-IL)(1)

Publication using 2769-IL

• Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, Kukova G, Buhl T, Ikoma A, Buddenkotte J, Soumelis V, Feld M, Alenius H, Dillon S, Carstens E, Homey B, Basbaum A, Steinhoff M A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1. J Allergy Clin Immunol, 2013-12-25;133(2):448-60. 2013-12-25 [PMID: 24373353] (IHC Control, Human)

Bioinformatics

• Gene Symbol: IL31RA
• Uniprot:
  • Q8NI17()