Recombinant Human IL-10 R alpha Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IL-10 R alpha Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-10-dependent proliferation of MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 6‑36 ng/mL
Source
Human embryonic kidney cell, HEK293-derived human IL-10 R alpha protein
Human IL-10 R alpha
(His22-Asn235)
Accession # Q13651
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
His22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL10RA
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
64-78 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-10 R alpha Fc Chimera Protein, CF

  • CD210 antigen
  • CDw210a
  • HIL-10R
  • IBD28
  • IL-10 R alpha
  • IL-10 receptor subunit alpha
  • IL10R alpha
  • IL-10R subunit 1
  • IL-10R1
  • IL10RA
  • IL-10Ra
  • IL10RIL-10R subunit alpha
  • interleukin 10 receptor, alpha
  • interleukin-10 receptor alpha chain
  • Interleukin-10 receptor subunit 1
  • interleukin-10 receptor subunit alpha

Background

Interleukin-10 Receptor alpha (IL-10 R alpha ), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer
(2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 R alpha consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 R alpha shares 59% aa sequence identity with mouse and rat IL-10 R alpha.
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
  3. Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
  4. Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
  5. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  6. Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
  7. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  8. Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
  9. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  10. Fernandez, S. et al. (2004) J. Immunol. 172:2613.
  11. Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
  12. Liu, Y. et al. (1994) J. Immunol. 152:1821.

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Bioinformatics

Gene Symbol IL10RA
Uniprot