Recombinant Human IGFBP-6 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGFBP-6 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.025-0.15 µg/mL in the presence of 14 ng/mL Recombinant Human IGF‑II (Catalog # 292-G2).
• Source: Mouse myeloma cell line, NS0-derived human IGFBP-6 protein
  Ala25-Gly240, with an N-terminal Met
• Accession #: NP_002169
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: IGFBP6
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGFBP-6 Protein, CF

• IBP6
• IBP-6
• IGF binding protein 6
• IGF-binding protein 6
• IGFBP6
• IGFBP-6
• insulin-like growth factor binding protein 6
• insulin-like growth factor-binding protein 6

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-6 cDNA encodes a 240 amino acid (aa) residue precursor protein with a putative 24 aa residue signal peptide that is processed to generate the 216 aa residue mature protein that is O-glycosylated. IGFBP-6 is expressed in ovarian cells, prostatic cells, and fibroblasts. IGFBP-6 is found predominantly in CSF and serum. IGFBP-6 binds preferentially to IGF-II, exhibiting a 2-fold higher affinity for IGF-II than for IGF-I.

1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Publications for IGFBP-6 (876-B6)(5)

Publications using 876-B6

• Q Chen, S Qin, Y Liu, M Hong, CN Qian, ET Keller, J Zhang, Y Lu IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker Oncotarget, 2016-10-18;7(42):68140-68150. 2016-10-18 [PMID: 27623076] (Bioassay, Human)
• Zhu W, Wu Y, Cui C, Zhao H, Ba J, Chen H, Yu J Expression of IGFBP6 in proliferative vitreoretinopathy rat models and its effects on retinal pigment epithelialJ cells. Mol Med Rep, 2013-11-12;9(1):33-8. 2013-11-12 [PMID: 24220750] (Bioassay, Rat)
• Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006-06-01;15(3):407-21. 2006-06-01 [PMID: 16846377] (Bioassay, Rat)
• Cortesio CL, Jiang W Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 2006-03-03;449(1):164-70. 2006-03-03 [PMID: 16554018] (Enzyme Assay, Human)
• Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6. 2005-08-05 [PMID: 15964556] (Bioassay, Enzyme Assay, Human)

Bioinformatics

• Gene Symbol: IGFBP6
• Uniprot:
  • NP_002169()