Recombinant Human HPRG Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human HPRG Protein, CF Summary

Details of Functionality
Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. Lamb-Wharton, R.J. and W.T. Morgen (1993) Cell Immunol. 152:544. When 1 x 105 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.4-2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human HPRG protein
Val19-Lys525, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Val19
Protein/Peptide Type
Recombinant Enzymes
Gene
HRG
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-85 kDa, reducing conditions
Publications
Read Publications using
1869-HP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HPRG Protein, CF

  • DKFZp779H1622
  • Histidine-proline-rich glycoprotein
  • histidine-rich glycoprotein
  • HPRG
  • HPRGHRGP
  • HRG

Background

Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67-75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules (aa 19-254) and one His-Pro-rich region (aa 350-497) that is flanked by two Pro-rich segments (aa 276-321 and 498-525) (3, 4). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++‑dependent manner, it is converted to plasmin by tPA (7-9). HPRG also shows antiangiogenic activity on endothelial cells (10).

  1. Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
  2. Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
  3. Koide, T. et al. (1986) Biochemistry 25:2220.
  4. SwissProt Accession # P04196.
  5. Borza, D-B. et al. (1996) Biochemistry 35:1925.
  6. Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
  7. Donate, F. et al. (2004) Cancer Res. 64:5812.
  8. Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
  9. Guan, X. et al. (2004) Thromb. Haemost. 92:403.
  10. Juarez, J.C. et al. (2002) Cancer Res. 62:5344.

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Publications for HPRG (1869-HP)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol HRG
Uniprot