Recombinant Human His6-GABARAP Fluorescein Protein, CF

Product Discontinued

Recombinant Human His6-GABARAP Fluorescein Protein, CF Summary

• Details of Functionality: Recombinant Human His6-GABARAP/Apg8p1 Fluorescein is ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 490 nM and an emission wavelength of 515 nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-GABARAP/Apg8p1 Fluorescein concentration of 0.1-1 μM.
• Source: E. coli-derived human GABARAP protein
  Contains an N-terminal 6-His tag
• Accession #: NP_009209
• Protein/Peptide Type: Recombinant Proteins
• Gene: GABARAP
• Purity: >95%, by PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity

Packaging, Storage & Formulations

• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer:

  X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl.Actual concentration varies with lot number.

• Purity: >95%, by PAGE

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-GABARAP Fluorescein Protein, CF

• Apg8p1
• ATG8A
• FLC3B
• FLJ25768
• GABA(A) receptor-associated proteinMGC120154
• GABARAP
• gamma-aminobutyric acid receptor-associated protein
• MM46
• MM46MGC120155

Background

gamma -Aminobutyric Acid Receptor-associated Protein (GABARAP), also known as Apg8p1, is 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins (1). The GABARAP subfamily also includes GABARAPL1. GABARAP/Apg8p1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAP/Apg8p1 was first described for its putative involvement GABA A Receptor trafficking (2,3). However, it is best known for its role in autophagy (1,4). GABARAP/Apg8p1 covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome maturation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
2. Wang, H. et al. (1999) Nature 397:69.
3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.

Bioinformatics

• Gene Symbol: GABARAP
• Uniprot:
  • NP_009209(Human)