Recombinant Human GST-KEAP1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GST-KEAP1 Protein, CF Summary

• Additional Information: Will be discontinued when existing inventory is gone.
• Details of Functionality: Typical protein concentration for use in vitro will depend on experimental conditions.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Keap1 protein
  Met1 - Cys624 with a N-terminal GST (glutathione S-transferase) tag
• Accession #: Q14145.2
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GST-KEAP1 Protein, CF

• INrf2
• INrf2MGC9454
• Keap1
• kelch-like ECH-associated protein 1
• Kelch-like protein 19
• KIAA0132MGC4407
• KLHL19Cytosolic inhibitor of Nrf2
• MGC10630
• MGC1114
• MGC20887

Background

Kelch-like ECH-associated protein (KEAP1) is the substrate recognition subunit of a BCR (BTB-CUL3-RBX) E3 ubiquitin ligase complex.  With a predicted molecular weight of 70 kDa, human KEAP1 IS 74% identical to its mouse and rat orthologues.  KEAP1 contains an N-terminal BB Domain that mediates interaction with CUL3, a central BACK Domain that plays a role in protein-protein interactions, and six C-terminal Kelch Domains that function in substrate recognition.  KEAP1 is an important sensor of oxidative and electrophilic stress.  Normally the KEAP1/CUL3/RBX1 complex ubiquitinates and causes degradation of NRF2, a transcription factor regulating expression of many cytoprotective genes.  Oxidative stress results in the inability of KEAP1 to ubiquitinate NRF2, leading to the latter proteins accumulation and subsequent upregulation of genes involved in cellular oxidative stress responses.  

1. Eggler, A.L. et al. (2009) Biochem. J. 422:171.
2. Eggler, A.L. et al. (2005) Proc. Natl. Acad. Sci. 102:10070.
3. Mills, E.L. et al. (2018) Nature  556:113. 
4. Wei, J. et al. (2021) J. Am. Chem. Soc. 143:15073.   

Bioinformatics

• Uniprot:
  • Q14145.2()