Recombinant Human gp130 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human gp130 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.5-2 µg/mL in the presence of 4 μg/mL recombinant human (rh) IL-6 R alpha and 25 pg/mL rhIL-6.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human gp130 protein
Leu24-Glu619 (Glu619Asp)
Accession #
N-terminal Sequence
Leu24
Protein/Peptide Type
Recombinant Proteins
Gene
IL6ST
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
228-GP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human gp130 Protein, CF

  • CD130 antigen
  • CD130
  • CDw130
  • DKFZp564F053
  • gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
  • gp130
  • IL-6 receptor subunit beta
  • IL-6R subunit beta
  • IL-6RB
  • IL-6R-beta
  • IL6ST
  • interleukin 6 signal transducer (gp130, oncostatin M receptor)
  • interleukin receptor beta chain
  • interleukin-6 receptor subunit beta
  • Interleukin-6 signal transducer
  • Membrane glycoprotein 130
  • membrane glycoprotein gp130
  • Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

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Publications for gp130/CD130 (228-GP/CF)(11)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 4 applications: Bioassay, ELISA capture, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(6)
ELISA capture
(2)
In Vivo
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
Rat
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 228-GP/CF Applications Species
H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression Cancer Res, 2017;0(0):. 2017 [PMID: 28235765] (Bioassay, Human) Bioassay Human
R Adams, RJ Burnley, CR Valenzano, O Qureshi, C Doyle, S Lumb, M Del Carmen, R Griffin, D McMillan, RD Taylor, C Meier, P Mori, LM Griffin, U Wernery, J Kinne, S Rapecki, TS Baker, AD Lawson, M Wright, A Ettorre Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling Sci Rep, 2017;7(0):37716. 2017 [PMID: 28134246] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016;90(0):88-95. 2016 [PMID: 27863335] (Bioassay, Human) Bioassay Human
Involvement of Spinal IL-6 Trans-Signaling in the Induction of Hyperexcitability of Deep Dorsal Horn Neurons by Spinal Tumor Necrosis Factor-Alpha J Neurosci, 2016;36(38):9782-91. 2016 [PMID: 27656018] (Bioassay, Rat) Bioassay Rat
Breakpoint Cluster Region-Mediated Inflammation Is Dependent on Casein Kinase II J Immunol, 2016;0(0):. 2016 [PMID: 27630163] (Bioassay, Human) Bioassay Human
Cho S, Pirih F, Koh A, Michalski M, Eber M, Ritchie K, Sinder B, Oh S, Al-Dujaili S, Lee J, Kozloff K, Danciu T, Wronski T, McCauley L The soluble interleukin-6 receptor is a mediator of hematopoietic and skeletal actions of parathyroid hormone. J Biol Chem, 2013;288(10):6814-25. 2013 [PMID: 23297399] (In Vivo, Mouse) In Vivo Mouse
Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor. J Biol Chem, 2012;287(39):32848-59. 2012 [PMID: 22829597] (ELISA capture, Human) ELISA capture Human
Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production. Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7. 2008 [PMID: 18796608] (Bioassay, Human) Bioassay Human
Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis. Arthritis Rheum., 2006;54(5):1662-72. 2006 [PMID: 16646038] (ELISA capture, Human) ELISA capture Human
Erices A, Conget P, Rojas C, Minguell JJ Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 2002;280(1):24-32. 2002 [PMID: 12372336] (Bioassay, Human) Bioassay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol IL6ST
Entrez
Uniprot