Recombinant Human gp130 His-tag Protein, CF

When Recombinant Human gp130 His-tag Protein (Catalog # 11067-GP) is immobilized at 0.5 µg/mL (100 µL/well) Recombinant Human IL-6 (7270-IL) in the presence of Recombinant Human IL-6R alpha (227-SR) binds with an ED50 of 0.200-3.00 ng/mL.

2 μg/lane of Recombinant Human gp130 His-tag Protein (Catalog # 11067-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-100 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human gp130 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human gp130 His-tag (Catalog # 11067-GP) is immobilized at 0.5 µg/mL (100 µL/well) Recombinant Human IL-6 (Catalog # 7270-IL) in the presence of Recombinant Human IL-6R alpha (Catalog # 227-SR) binds with an ED₅₀ of 0.200-3.00 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human gp130 protein
  Glu23-Ile618, with a C-terminal 6-His tag
• Accession #: AAA59155.1
• N-terminal Sequence: Glu23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 86-100 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human gp130 His-tag Protein, CF

• CD130 antigen
• CD130
• CDw130
• DKFZp564F053
• gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• gp130
• IL-6 receptor subunit beta
• IL-6R subunit beta
• IL-6RB
• IL-6R-beta
• IL6ST
• interleukin 6 signal transducer (gp130, oncostatin M receptor)
• interleukin receptor beta chain
• interleukin-6 receptor subunit beta
• Interleukin-6 signal transducer
• Membrane glycoprotein 130
• membrane glycoprotein gp130
• Oncostatin-M receptor subunit alpha

Background

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Human gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 597 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 277 aa cytoplasmic tail. The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (1-2). The ECD of human gp130 shares 73% and 75% aa sequence identity with that of mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)‑6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2 - 5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2). 

1. Wang, Y. et al. (1992) Genomics 14:666.
2. Muller-Newen, G. (2003) Sci. STKE pe40.
3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.

Bioinformatics

• Uniprot:
  • AAA59155.1()