Recombinant Human Frizzled-10 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Frizzled-10 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an estimated Kd <5 nM.
Source
Mouse myeloma cell line, NS0-derived human Frizzled-10 protein
Human Frizzled-10
(Ile21-Gly161)
Accession # Q6NSL8
IEGRMD IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FZD10
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
43 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
56-58 kDa, reducing conditions
Publications
Read Publication using
3459-FZ in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Frizzled-10 Fc Chimera Protein, CF

  • CD350 antigen
  • CD350
  • frizzled (Drosophila) homolog 10
  • frizzled homolog 10 (Drosophila)
  • Frizzled10
  • Frizzled-10
  • Fz10
  • FZ-10
  • FZD10
  • FzE7
  • hFz10

Background

Frizzled-10, also known as CD350, is a 68 kDa seven pass transmembrane glycoprotein in the Frizzled family of Wnt receptors (1, 2). The 205 amino acid N-terminal extracellular region of Frizzled-10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization (3‑5). The C-terminal cytoplasmic tail contains a PDZ-interaction motif (3). PDZ motifs mediate intracellular binding to scaffolding proteins. Within the cysteine-rich domain, human Frizzled‑10 shares 71% amino acid (aa) sequence identity with Frizzled-9 and 31%-46% with Frizzled-1, -2, -3, -4, -5, - 6, -7, and -8. It shares 96%, 94%, 90%, and 82% aa sequence identity with chick, mouse, Xenopus, and zebrafish Frizzled-10, respectively. Frizzled-10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud, and airway epithelium (6-11). It is induced by Shh and colocalizes with Shh and Wnt-7a in the neural tube (12, 13). In the adult, Frizzled-10 is expressed in placenta, gastric glands, and colon and renal tubule epithelial cells (4). Frizzled-10 associates with LRP5 to transduce Wnt-7a and Wnt-7b signals, resulting in the stabilization of cytoplasmic beta-catenin (11, 13). Frizzled-10 is also up-regulated in some cancers and transformed cell lines (4, 14). It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas (15).

  1. Kikuchi, A. et al. (2007) Cell. Signal. 19:659.
  2. Wang, H. et al. (2006) Cell. Signal. 18:934.
  3. Koike, J. et al. (1999) Biochem. Biophys. Res. Commun. 262:39.
  4. Nagayama, S. et al. (2005) Oncogene 24:6201.
  5. Dann, C.E. et al. (2001) Nature 412:86.
  6. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  7. Kawakami, Y. et al. (2000) Mech. Dev. 91:375.
  8. Nasevicius, A. et al. (2000) Mech. Dev. 92:311.
  9. Moriwaki, J. et al. (2000) Biochem. Biophys. Res. Commun. 278:377.
  10. Stark, M.R. et al. (2000) Mech. Dev. 93:195.
  11. Wang, Z. et al. (2005) Mol. Cell. Biol. 25:5022.
  12. Nunnally, A.P. and B.A. Parr (2004) Dev. Genes Evol. 214:144.
  13. Kawakami, Y. et al. (2000) Dev. Growth Differ. 42:561.
  14. Saitoh, T. et al. (2002) Int. J. Oncol. 20:117.
  15. Togashi, A. et al. (2005) Cancer Res. 65:4817.

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Publications for Frizzled-10 (3459-FZ)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol FZD10
Entrez
Uniprot