Recombinant Human FOLR2 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human FOLR2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Folic Acid-Bovine Serum Albumin was coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human FOLR-2 that produces 50% of the optimal binding response was found to be <1 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human FOLR2 protein
  Gln22-His228, with a C-terminal 6-His tag
• Accession #: P14207
• N-terminal Sequence: No results obtained: Gln22 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: FOLR2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 25.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FOLR2 Protein, CF

• beta-HFR
• FBP
• FBP/PL-1
• folate receptor 2 (fetal)
• Folate receptor 2
• folate receptor beta
• folate receptor, beta
• Folate receptor, fetal/placental
• folate-binding protein, fetal/placental
• FOLR2
• FR-beta
• FR-P3
• Placental folate-binding protein

Background

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4 - 6). It is upregulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8 - 10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
3. Shen, F. et al. (1994) Biochemistry 33:1209.
4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
5. Ross, J.F. et al. (1999) Cancer 85:348.
6. Reddy, J.A. et al. (1999) Blood 93:3940.
7. Ross, J.F. et al. (1994) Cancer 73:2432.
8. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
9. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
10. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
11. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
12. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Bioinformatics

• Gene Symbol: FOLR2
• Uniprot:
  • P14207(Human)