Recombinant Human FLRT3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human FLRT3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. Recombinant Human FLRT3 immobilized at 5 μg/mL, 100 μL/well, will mediate >50% Neuro‑2A cell adhesion.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human FLRT3 protein
  Lys29-Pro528, with a C-terminal 6-His tag
• Accession #: Q9NZU0
• N-terminal Sequence: Lys29
• Protein/Peptide Type: Recombinant Proteins
• Gene: FLRT3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57.3 kDa .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-90 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FLRT3 Protein, CF

• fibronectin leucine rich transmembrane protein 3
• Fibronectin-like domain-containing leucine-rich transmembrane protein 3
• FLRT3
• HH21
• KIAA1469
• leucine-rich repeat transmembrane protein FLRT3

Background

FLRT3 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85-95 kDa type I transmembrane (TM) human FLRT3 is synthesized as a 649 amino acid (aa) precursor with a 28 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa TM segment and a 100 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT3 ECD shares 96%, 96%, 97%, 97%, 98% and 81% aa sequence identity with mouse, rat, canine, bovine, equine and Xenopus FLRT3 ECD, respectively, and 61% and 48% aa identity to human FLRT2 and FLRT3 ECDs, respectively. The fibronectin domain is responsible for binding to FGF receptors, and is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (4). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (4, 5). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (2). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (2). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (6). Postnatally, FLRT3 mRNA is widely expressed (1). It is up-regulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8).

 

1. Lacy, S. E. et al. (1999) Genomics 62:417.
2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
6. Maretto, S. et al. (2008) Dev. Biol. 318:184.
7. Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
8. Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.

Publications for FLRT3 (2795-FL)(2)

Publications using 2795-FL

• Teramoto, Y;Elahi Najafi, MA;Matsukawa, T;Sharma, A;Goto, T;Miyamoto, H; Latrophilins as Downstream Effectors of Androgen Receptors including a Splice Variant, AR-V7, Induce Prostate Cancer Progression International journal of molecular sciences 2024-07-02 [PMID: 39000396] (Bioassay, Human)
• M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats J. Neurosci., 2019-07-25;0(0):. 2019-07-25 [PMID: 31346030] (Bioassay, ELISA Capture, In Vivo, Human, Rat)

Bioinformatics

• Gene Symbol: FLRT3
• Uniprot:
  • Q9NZU0()