Recombinant Human Fc alpha/mu R His-tag Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Fc alpha/mu R His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Human IgM is immobilized at 2.5 μg/mL, 100 μL/well, the concentration of Recombinant Human Fc alpha /μ R that produces 50% of the optimal binding response is approximately 0.12-0.72 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Fc alpha/mu R protein
  Leu17-Arg450, with a C-terminal 6-His tag
• Accession #: Q8WWV6
• N-terminal Sequence: Leu17
• Protein/Peptide Type: Recombinant Proteins
• Gene: FCAMR
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 84-108 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc alpha/mu R His-tag Protein, CF

• CD351 antigen
• CD351
• Fc alpha/mu R
• Fc alpha/mu receptor
• Fc receptor, IgA, IgM, high affinity
• FCA/MR
• FCAMR
• FKSG87
• high affinity immunoglobulin alpha and immunoglobulin mu Fc receptor
• immunity related factor
• receptor for Fc fragment of IgA and IgM
• uR

Background

Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60 kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature human FCAMR consists of a 434 amino acid (aa) extracellular domain with one Ig-like domain, a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain (2). Within the ECD, human FCAMR shares 51% aa sequence identity with mouse and rat FCAMR. Alternative splicing generates additional isoforms that are truncated following the Ig-like domain, are truncated before the transmembrane segment, or carry a 9 aa deletion and a substitution of the transmembrane segment. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).

1. Wang, H. et al. (2016) Front. Immunol. 7:99.
2. Shibuya, A. et al. (2000) Nat. Immunol. 1:441.
3. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
4. McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438.
5. Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147.
6. Feng, X. et al. (2010) Atherosclerosis 208:396.

Bioinformatics

• Gene Symbol: FCAMR
• Uniprot:
  • Q8WWV6()