Recombinant Human Epigen Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Epigen Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is ≤ 300 ng/mL.
• Source: E. coli-derived human Epigen protein
  Leu54-Ala104, with an N-terminal Met
• Accession #: Q6UW88
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPGN
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 5.5 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Epigen Protein, CF

• ALGV3072
• EPG
• EPGN
• Epigen
• PRO9904

Background

Epigen (EPGN) is an epithelial mitogen that belongs to the EGF superfamily (1 ‑ 3). Human Epigen cDNA encodes a 154 amino acid (aa) residue type I transmembrane precursor glycoprotein with a 22 aa signal peptide, an 88 aa extracellular domain, a 21 aa transmembrane domain and a 23 aa cytoplasmic domain (2, 3). The extracellular domain (aa 23 ‑ 110) contains a pattern of six cysteines typical of EGF family proteins (1 ‑ 3). Potential splice variants of 133, 112, 103, 94 or 73 aa, lacking either N‑terminal sequence (aa 15 or 36 to 44), transmembrane and juxtamembrane sequence (aa 87 or 96 to 137), or sequences in both regions, have been described (4). In addition to potentially secreted splice variants, Epigen is among several EGF family proteins that can undergo metalloproteinase cleavage to generate a soluble form (3, 5). Cleavage of Epigen by TACE/ADAM17 has been demonstrated (5). The mature, shed form of human Epigen (aa 54 ‑ 104) shares 92%, 94% and 94% aa sequence identity with mouse, rat and equine Epigen, respectively, and more than 40% aa identity with corresponding regions of TGF‑ alpha and epiregulin (2). Epigen mRNA is found in many tissues, but it is mainly expressed in actively proliferating cells within these tissues. For example, Epigen in skin is found mainly in the proliferating root sheath of hair follicles, and transgenic overexpression in the skin causes hyperplasia in sebaceous glands (3, 6). Epigen is also expressed developmentally and by invasive adenocarcinomas of the breast and prostate (3). Epigen is strongly mitogenic for epithelial cells and fibroblasts, despite its relatively weak affinity for its main receptor, ErbB1 (2, 3). The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor‑mediated depletion (3).

1. Schneider, M.R. and E. Wolf (2010) J. Cell. Physiol. 218:460.
2. Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
3. Kochupurakkal, B.S. et al. (2005) J. Biol. Chem. 280:8503.
4. SwissProt Accession # Q6UW88.
5. Sahin, U. and C.P. Blobel 2007) FEBS Lett. 581:41.
6. Dahlhoff, M. et al. (2010) J. Invest. Dermatol. 130:623.

Publications for Epigen (6629-EP/CF)(1)

Publication using 6629-EP/CF

• C Hu, CA Leche, A Kiyatkin, Z Yu, SE Stayrook, KM Ferguson, MA Lemmon Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias Nature, 2022-02-09;602(7897):518-522. 2022-02-09 [PMID: 35140400] (Bioassay, Human)

Bioinformatics

• Gene Symbol: EPGN
• Uniprot:
  • Q6UW88()