Recombinant Human DSPG3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DSPG3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin. When 5 x 10 4 cells/well are added to Human DSPG3 and Recombinant Human Fibronectin (0.5 μg/mL, (Catalog # 4305-FNB) coated plates, the cell adhesion is enhanced in a dose dependent manner. The ED 50 for this effect is 0.05-1 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human DSPG3 protein Met1-Val322 with a C-terminal 10-His tag
• Accession #: Q99645
• N-terminal Sequence: Ala20
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPYC
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DSPG3 Protein, CF

• Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3
• DSPG3
• DSPG3proteoglycan-lb
• Epiphycan
• EPYC
• PGLB
• PG-Lb
• Proteoglycan-Lb
• SLRR3B
• SLRR3Bepiphycan proteoglycan
• Small chondroitin/dermatan sulfate proteoglycan

Background

Human DSPG3 (dermatan sulfate proteoglycan 3), also called epiphycan (gene name EPYC) or PG-Lb, is a 95-100 kDa class III subfamily member of the SLRP (small leucine-rich proteoglycans) family of molecules (1-4). It is synthesized as a 322 amino acid (aa) precursor that contains a 19 aa signal sequence and a 303 aa mature region. The mature molecule contains three N-terminal O-glycosylation sites (including one found to bind dermatin sulfate) and a glutamic acid-rich motif followed by six leucine-rich repeats (LRR) that contain two potential N-glycosylation sites (2, 3). The LRR are flanked by two intrachain disulfide bonds (5). Class III SLRPs, including DSPG3, Osteoglycin and Opticin, contain 6-7 LRR, while other classes contain 8 - 10 LRR (1, 4). Collagen binding maps to the LRR (1). Mature human DSPG3 shares 84%, 85%, 95% and 96% aa sequence identity with mouse, rat, bovine and canine DSPG3, respectively. DSPG3 is found predominantly in epiphyseal cartilage, although DSPG3 mRNA is also detected in mouse testis and human ligament and placenta (2-4). In mouse, expression begins in mid-gestation as an intermediate marker for chondrogenesis, peaks at birth and declines thereafter (6, 7). Deletion of DSPG3 in mouse results in a mild postnatal phenotype that worsens synergistically when the co-expressed SLRP biglycan is also deleted (7). The double-mutant phenotype includes premature osteoarthritis, indicating a role for DSPG3, in cooperation with other SLRPs, in stability of the collagen matrix and maintenance of joint integrity (7).

1. Kalamajski, S. and A. Oldberg (2010) Matrix Biol. 29:248.
2. Kurita, K. et al. (1996) Biochem. J. 318:909.
3. Deere, M. et al. (1996) Genomics 38:399.
4. Deere, M. et al. (1999) Genome Res. 9:449.
5. Swiss-Prot Accession # Q96645 and P70186.
6. Johnson, J. et al. (1999) Dev. Dyn. 216:499.
7. Nuka, S. et al. (2010) Osteoarthritis Cartilage 18:88.

Bioinformatics

• Gene Symbol: EPYC
• Uniprot:
  • Q99645()