Recombinant Human Cryptic Protein, CF


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Cryptic Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326. The ED50 for this effect is 1.5-7.5 μg/mL. Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos. 80 ng per embryo induces 25-50% heart reversals.
Mouse myeloma cell line, NS0-derived human Cryptic protein
Human Cryptic
(Tyr26 - Gly169)
Accession # NP_115934
Asp10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
17.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
24 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Cryptic Protein, CF

  • CFC1
  • CFC-1
  • CFC1B
  • cripto, FRL-1, cryptic family 1
  • Cryptic family protein 1
  • cryptic protein
  • Cryptic
  • HTX2MGC133213


Cryptic, also known at CFC-1, was originally identified as a secreted protein that is specifically expressed during mesoderm differentiation (1). Cryptic, along with Cripto, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead) define the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes (2, 3). Overall sequence identity between members of the family is low, but they do share distinct domains: a variant EGF-like motif, a novel conserved cysteine-rich domain (called CFC domain), and a C-terminal hydrophobic region (2). Most EGF-CFC members have a glycosyl‑phosphatidylinositol (GPI) anchoring site at the C‑terminus and exist as extracellular membrane‑anchored proteins. However, naturally‑occurring soluble isoforms also exist. Human Cryptic shares 55% and 25% amino acid idenitity with mouse Cryptic and human Cripto, respectively. Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2).

Cryptic is expressed during gastrulation in the mesoderm and later in the neuroectoderm, marking the prospective floor plate of the neural tube (1). Genetic evidence from mice and humans points to a role for Cryptic in determining left‑right asymmetry. Mutations in the cryptic gene result in a spectrum of heart, lung and spleen defects, all representing left‑right laterality defects (4, 5). These phenotypes resemble some Nodal mutant alleles suggesting that Cryptic, like Cripto, acts as an essential cofactor for Nodal signaling (1, 3). Studies have shown that other TGF-beta superfamily members involved in mesoderm induction and left‑right patterning, Vg1 and GDF-1, also require EGF-CFC cofactors. Cryptic binds to GDF-1 leading to an Act RIIB-ALK4-Cryptic-GDF-1 complex for signaling (6).

  1. Shen, M. et al. (1997) Development 124:429. 
  2. Shen, M. and A. Schier (2000) Trends Genet. 16:303.
  3. Rosa, F.M. (2002) Science's STKE
  4. Gaio, U. et al. (1999) Curr. Biol. 9:1339.
  5. Bamford, R. et al. (2000) Nature Genet. 26:365.
  6. Cheng, S. et al. (2003) Genes & Dev. 17:31.

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Gene Symbol CFC1