Recombinant Human Contactin-6 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Contactin-6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED 50 for this efffect is 1.0-4.0 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Contactin-6 protein

  Human Contactin-6 (Asp20-Ser891) Accession # Q9UQ52

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q9UQ52
• N-terminal Sequence: Asp20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CNTN6
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 122.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-170 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Contactin-6 Fc Chimera Protein, CF

• CNTN6
• contactin 6
• Contactin6
• Contactin-6
• MGC133256
• NB3

Background

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature human Contactin-6 consists of six immunoglobulin-like domains (aa 26-587), a flexible linker region, four fibronectin type III domains (aa 597-992), and a GPI anchor (3). Within aa 20-891, human Contactin-6 shares 89% aa sequence identity with mouse and rat Contactin-6. Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3-7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1, PTPR alpha, and PTPR gamma as well as in trans with Notch-1 on oligodendrocytes (6, 8-10). Its binding to Notch-1 triggers the release and translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
2. Stoeckli, E.T. (2010) Cell Adh. Migr. 4:523.
3. Kamei, Y. et al. (1998) J. Neurosci. Res. 51:275.
4. Lee, S. et al. (2000) Gene 245:253.
5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
7. Sakurai, K. et al. (2009) Dev. Neurobiol. 69:811.
8. Ye, H. et al. (2008) EMBO J. 27:188.
9. Bouyain, S. and D.J. Watkins (2010) Proc. Natl. Acad. Sci. 107:2443.
10. Ye, H. et al. (2011) J. Biol. Chem. 286:26071.

Bioinformatics

• Gene Symbol: CNTN6
• Uniprot:
  • Q9UQ52(Human)