Recombinant Human Contactin-1 Fc Chimera Protein, CF

Images

 
There are currently no images for Contactin-1 (904-CN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Contactin-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) NrCAM Fc Chimera (Catalog # 2034-NR) at 1 μg/mL (100 μL/well) can bind rhContactin-1 Fc Chimera with a linear range of 0.3-20 ng/mL. Measured by its ability to support the adhesion of C6 rat glioma cells. rhContactin Fc Chimera immobilized at 2 µg/mL, 100 µL/well will mediate >20% C6 cell adhesion.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Contactin-1 protein
Human Contactin-1
(Glu21-Ser993)
Accession # CAA79696
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CNTN1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
135-150 kDa, reducing conditions
Publications
Read Publication using
904-CN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Contactin-1 Fc Chimera Protein, CF

  • CNTN1
  • contactin 1
  • Contactin1
  • Contactin-1
  • F11
  • F3
  • F3cam
  • Glycoprotein gp135
  • GP135
  • Neural cell surface protein F3

Background

Contactin-1 (CNTN1), also known as F3 and F11, is an approximately 135 kDa GPI-anchored member of the Contactin family of neuronal adhesion glycoproteins (1). Mature human Contactin-1 contains 6 Ig-like domains and 4 fibronectin type III-like domains, and it shares 96% amino acid (aa) sequence identity with mouse and rat Contactin-1 (2, 3). Alternative splicing generates an isoform with an 11 aa deletion N-terminal to the first Ig-like domain and another isoform that is substituted and truncated following the sixth Ig-like domain. A soluble form of Contactin-1 is released into the cerebrospinal fluid (4). Contactin-1 is expressed on neurons and their precursors at sites of glial cell contact with neuronal processes (5-7). Within paranodal regions of the axon, Contactin-1 associates in cis with the transmembrane protein Caspr (7). It binds in trans to other neuronal adhesion proteins (e.g. NrCAM, Neurofascin, Tenascin R), the phosphatase PTPRZ, and the chondroitin sulfate CS-E (8-13). It also binds and activates both Notch-1 and Notch-2 (14). Ligation of Contactin-1 promotes neurite outgrowth, adhesion to Schwann cells, axon myelination, and differentiation of oligodendrocytes and cerebellar granule neurons, but it can also negatively regulate neurogenesis in the ventricular zone
(4, 6-9, 12-14). In cancer, Contactin-1 is up-regulated by VEGF-C and is required for VEGF R3/Flt-4 induced tumor cell invasion and metastasis (15).
  1. Shimoda, Y. and K. Watanabe (2009) Cell Adh. Migr. 3:64.
  2. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
  3. Berglund, E.O. and B. Ranscht (1994) Genomics 21:571.
  4. Durbec, P. et al. (1992) J. Cell Biol. 117:877.
  5. Gennarini, G. et al. (1990) Acta Histochem. 38:65.
  6. Bizzoca, A. et al. (2012) Dev. Biol. 365:133.
  7. Boyle, M.E.T. et al. (2001) Neuron 30:385.
  8. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  9. Xenaki, D. et al. (2011) Development 138:519.
  10. Volkmer, H. et al. (1998) J. Cell Biol. 142:1083.
  11. Pesheva, P. et al. (1993) Neuron 10:69.
  12. Peles, E. et al. (1995) Cell 82:251.
  13. Mikami, T. et al. (2009) J. Biol. Chem. 284:4494.
  14. Hu, Q.-D. et al. (2003) Cell 115:163.
  15. Su, J.-L. et al. (2006) Cancer Cell 9:209.

Customers Who Viewed This Item Also Viewed...

NBP1-58268
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00008458-M06
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP, KD
NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-84957
Species: Hu
Applications: WB, IHC, IHC-P
H00000518-P01
Species: Hu
H00201780-P01
Species: Hu
NB100-91761
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-67429
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF2168
Species: Hu, Mu
Applications: WB, Simple Western, ChIP
NBP2-57642
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-15514
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow, KO
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Contactin-1 (904-CN)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Contactin-1 (904-CN) (0)

There are no reviews for Contactin-1 (904-CN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Contactin-1 (904-CN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Contactin-1 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CNTN1
Uniprot