Recombinant Human Contactin-1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity, Bioactivity
• Format: Carrier-Free

Recombinant Human Contactin-1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) NrCAM Fc Chimera (Catalog # 2034-NR) at 1 μg/mL (100 μL/well) can bind rhContactin-1 Fc Chimera with a linear range of 0.3-20 ng/mL.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Contactin-1 protein
  

  Human Contactin-1 (Glu21-Ser993) Accession # CAA79696	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: CAA79696
• N-terminal Sequence: Glu21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CNTN1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity2
  • Bioactivity
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Contactin-1 Fc Chimera Protein, CF

• CNTN1
• contactin 1
• Contactin1
• Contactin-1
• F11
• F3
• F3cam
• Glycoprotein gp135
• GP135
• Neural cell surface protein F3

Background

Contactin-1 (CNTN1), also known as F3 and F11, is an approximately 135 kDa GPI-anchored member of the Contactin family of neuronal adhesion glycoproteins (1). Mature human Contactin-1 contains 6 Ig-like domains and 4 fibronectin type III-like domains, and it shares 96% amino acid (aa) sequence identity with mouse and rat Contactin-1 (2, 3). Alternative splicing generates an isoform with an 11 aa deletion N-terminal to the first Ig-like domain and another isoform that is substituted and truncated following the sixth Ig-like domain. A soluble form of Contactin-1 is released into the cerebrospinal fluid (4). Contactin-1 is expressed on neurons and their precursors at sites of glial cell contact with neuronal processes (5-7). Within paranodal regions of the axon, Contactin-1 associates in cis with the transmembrane protein Caspr (7). It binds in trans to other neuronal adhesion proteins (e.g. NrCAM, Neurofascin, Tenascin R), the phosphatase PTPRZ, and the chondroitin sulfate CS-E (8-13). It also binds and activates both Notch-1 and Notch-2 (14). Ligation of Contactin-1 promotes neurite outgrowth, adhesion to Schwann cells, axon myelination, and differentiation of oligodendrocytes and cerebellar granule neurons, but it can also negatively regulate neurogenesis in the ventricular zone
(4, 6-9, 12-14). In cancer, Contactin-1 is up-regulated by VEGF-C and is required for VEGF R3/Flt-4 induced tumor cell invasion and metastasis (15).

1. Shimoda, Y. and K. Watanabe (2009) Cell Adh. Migr. 3:64.
2. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
3. Berglund, E.O. and B. Ranscht (1994) Genomics 21:571.
4. Durbec, P. et al. (1992) J. Cell Biol. 117:877.
5. Gennarini, G. et al. (1990) Acta Histochem. 38:65.
6. Bizzoca, A. et al. (2012) Dev. Biol. 365:133.
7. Boyle, M.E.T. et al. (2001) Neuron 30:385.
8. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
9. Xenaki, D. et al. (2011) Development 138:519.
10. Volkmer, H. et al. (1998) J. Cell Biol. 142:1083.
11. Pesheva, P. et al. (1993) Neuron 10:69.
12. Peles, E. et al. (1995) Cell 82:251.
13. Mikami, T. et al. (2009) J. Biol. Chem. 284:4494.
14. Hu, Q.-D. et al. (2003) Cell 115:163.
15. Su, J.-L. et al. (2006) Cancer Cell 9:209.

Publications for Contactin-1 (904-CN)(1)

Publication using 904-CN

• Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenco T, dos Santos F, Andrade P, Cardoso C, Vieira M, Paiva A, da Silva C, Cabral J, Relvas J, Graos M Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage. PLoS ONE, 2014-10-30;9(10):e111059. 2014-10-30 [PMID: 25357129] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CNTN1
• Uniprot:
  • CAA79696()