Recombinant Human Complement Factor H-related 4/CFHR4, CF

Images

 
2 μg/lane of Recombinant Human Complement Factor H‑related 4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at ~42 kDa and ...read more
When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL, 100 µL/well, Recombinant HumanComplement Factor H-related 4/CFHR4 (Catalog # 5980‑CH) binds with an ED50 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Complement Factor H-related 4/CFHR4, CF Summary

Additional Information
His-tag
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 binds with an ED50 of 1-6 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Complement Factor H-related 4/CFHR4 protein
Gln19-Glu331, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Glu20.
Protein/Peptide Type
Recombinant Enzymes
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
39-45 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Factor H-related 4/CFHR4, CF

  • complement factor H-related protein 4
  • CFHL4
  • CFHR4
  • Complement Factor Hrelated 4
  • Complement Factor H-related 4
  • FHR4
  • FHR-4

Background

Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5). CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The longer 86 kDa alternative splice form termed FHR4A includes a duplication insertion of 247 aa. The CFHR4 product represents the shorter isoform, FHR4B, containing 5 of 9 SCRs (6).
  1. Skerka, C. et al. (2013) Mol. Immunol. 56:170.
  2. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
  3. Hellwage, J. et al. (1999) FEBS Lett. 462:345.
  4. Hebecker, M. and J. Jozsi (2011) J. Biol. Chem. 287:19528.
  5. Mihlan, M. et al. (2009) Mol. Immunol. 46:335.
  6. Jozsi, M. et al. (2005) Eur. J. Hum. Genet. 13:321.

Customers Who Viewed This Item Also Viewed...

AF4999
Species: Mu
Applications: WB, Simple Western
NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB4247
Species: Hu
Applications: WB, IP
NBP2-92615
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-52441
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA
NBP1-31336
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB5484
Species: Hu
Applications: WB, IP
NBP1-89985
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
MAB5938
Species: Hu
Applications: WB, ICC
NBP1-52157
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA
NBP2-70997
Species: Hu
Applications: WB, IHC
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP1-89811
Species: Hu
Applications: WB, IHC, IHC-P
AF2005
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
5980-CH
Species: Hu
Applications: Bioactivity

Publications for Complement Factor H-related 4/CFHR4 (5980-CH) (0)

There are no publications for Complement Factor H-related 4/CFHR4 (5980-CH).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Complement Factor H-related 4/CFHR4 (5980-CH) (0)

There are no reviews for Complement Factor H-related 4/CFHR4 (5980-CH). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Complement Factor H-related 4/CFHR4 (5980-CH) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Complement Factor H-related 4/CFHR4, CF and receive a gift card or discount.

Bioinformatics

Uniprot