Recombinant Human Common beta Chain His-tag Protein, CF

In the presence of Recombinant Human GM‑CSF R alpha (Catalog # 706-GR), Recombinant Human Common Chain binds to Recombinant Human GM‑CSF (Catalog # 215-GM) with an ED50 of 20-120 ng/mL.

2 μg/lane of Recombinant Human Common beta Chain was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 57-65 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Common beta Chain His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Common beta Chain binds Recombinant Human GM‑CSF (Catalog # 215-GM) in the presence of Recombinant Human GM‑CSF R alpha (Catalog # 706-GR). The concentration of Recombinant Human Common beta that produces 50% of the optimal binding response is 20-120 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Common beta Chain protein
  Trp17-Trp443, with a C-terminal 6-His tag
• Accession #: P32927-1
• N-terminal Sequence: Gly23 & Glu25
• Protein/Peptide Type: Recombinant Proteins
• Gene: CSF2RB
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 57-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Common beta Chain His-tag Protein, CF

• beta, low-affinity
• CD131 antigen
• CD131
• CDw131
• colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage)
• Common beta Chain
• CSF2RB
• cytokine receptor common subunit beta
• GM-CSF R beta
• GM-CSF/IL-3/IL-5 receptor common beta subunit
• GM-CSF/IL-3/IL-5 receptor common beta-chain
• IL-3 R beta
• IL3RB
• IL-5 R beta
• interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)

Background

The common beta chain (beta c) is also known as GM-CSF R beta (granulocyte-macrophage colony stimulating factor receptor beta subunit), IL-3 R beta, and IL-5 R beta (gene name CSF2RB and designated CD131) (1, 2). It is a 58-61 kDa type I transmembrane protein that associates with the ligand-specific IL-3 R alpha on T cells and other cells, IL-5 R alpha on eosinophils or GM-CSF R alpha on myeloid cells, to form high affinity receptor complexes (1, 2). The 897 amino acid (aa) human beta c contains a 16 aa signal sequence, a 427 aa extracellular domain (ECD) with two fibronectin type III domains, a transmembrane sequence, and a 437 aa cytoplasmic domain (2, 3). Within the ECD, human beta c shares 57-68% aa sequence identity with mouse, rat, equine, porcine, bovine and canine beta c. Complexes of GM-CSF with its specific alpha subunit then bind preformed beta c dimers, creating 2:2:2 hexamers that can combine to a dodecamer (4:4:4) (3). Beta c association and dimerization via the dimerization of the hexamer to the dodecamer is essential for JAK2 activation via GM-CSF signaling which imparts growth and survival signals (3). Except for eosinophils, beta c is primarily involved when rapid production of leukocytes is needed, rather than for developmental or steady-state cell production (1). Beta c also associates with other receptors, forming heteroreceptor complexes that allow beta c complexes to influence the signaling pathways activated by the associated receptor (1). Beta c thus enhances angiogenesis (when associated with VEGF R2/KDR/Flt-1 or beta 1 integrins), cell protection (with Erythropoietin R), and synergistic growth of stem cells (with SCF R/c- kit) (4-9). Defective production of beta c in humans is a cause of pulmonary alveolar proteinosis (10).

1. Lopez, A.F. et al. (2010) IUBMB Life 62:509.
2. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
3. Hansen, G. et al. (2008) Cell 134:496.
4. Blake, T.J. et al. (2002) J. Leukoc. Biol. 72:1246.
5. Brines, M. et al. (2004) Proc. Natl. Acad. Sci. USA 101:14907.
6. Dentelli, P. et al. (2005) Oncogene 24:6394.
7. Saulle, E. et al. (2009) Br. J. Haematol. 145:399.
8. Lennartsson, J. et al. (2004) J. Biol. Chem. 279:44544.
9. Uberti, B. et al. (2010) Oncogene 29:6581.
10. Dirksen, U. et al. (1997) J. Clin. Invest. 100:2211.

Bioinformatics

• Gene Symbol: CSF2RB
• Uniprot:
  • P32927-1()