Recombinant Human CLEC17A Fc Chimera Protein, CF

Immobilized Recombinant Human CLEC17A Fc Chimera (Catalog #10147-CL) supports the adhesion of the MCF‑7 human breast cancer cells. TheED50 for this effect is 1.5-9 μg/mL.

2 μg/lane of Recombinant Human CLEC17A Fc Chimera (Catalog # 10147-CL) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at 54-66 kDa and 110-130 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CLEC17A Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 1.5-9 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CLEC17A protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Mouse CLEC17A (Lys194-Cys378) Accession # Q6ZS10-1
  N-terminus			C-terminus

• Accession #: Q6ZS10-1
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-66 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CLEC17A Fc Chimera Protein, CF

• CLEC17A
• C-type lectin domain family 17, member A
• Prolectin

Background

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2).  It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca²⁺-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

1. Graham, S. A. et al. (2009) J. Biol. Chem. 284:18537.
2. Breiman, A. et al. (2016) Oncotarget 7:14064.
3. Koh, G. et al. (2011) BMC Bioinformatics 12:S5.
4. Zelensky AN. et al. (2005) FEBS Journal 272:6179.

Bioinformatics

• Uniprot:
  • Q6ZS10-1()