Recombinant Human CDNF Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CDNF Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 6-24 µg/mL on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human CDNF protein
  Gln25-Leu187, with a C-terminal 10-His tag
• Accession #: Q49AH0
• N-terminal Sequence: No results obtained: Gln25 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: CDNF
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19-25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CDNF Protein, CF

• arginine-rich, mutated in early stage tumors-like 1
• ARMETL1
• ARMET-like protein 1
• CDNF
• cerebral dopamine neurotrophic factor
• Conserved dopamine neurotrophic factor

Background

CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1) in mouse, is a 17 - 19 kDa secreted protein that shares 62% amino acid (aa) identity with human MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet in mouse (1). The Armet designation is not preferred, because the proteins when translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfides, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1 - 3). The human CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature human CDNF shares 80%, 84%, 90% and 92% aa identity with mouse, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre- and post-natal mouse brain, they are most abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson’s disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).

1. Lindholm, P. et al. (2007) Nature 448:73.
2. Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
3. Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
4. Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.

Publications for CDNF (5097-CD)(2)

Publications using 5097-CD

• X Liu, H Ren, A Peng, H Cheng, J Chen, X Xia, T Liu, X Wang The Effect of RADA16-I and CDNF on Neurogenesis and Neuroprotection in Brain Ischemia-Reperfusion Injury International Journal of Molecular Sciences, 2022-01-27;23(3):. 2022-01-27 [PMID: 35163360] (Bioassay, Rat)
• GL Zhang, LH Wang, XY Liu, YX Zhang, MY Hu, L Liu, YY Fang, Y Mu, Y Zhao, SH Huang, T Liu, XJ Wang Cerebral Dopamine Neurotrophic Factor (CDNF) Has Neuroprotective Effects against Cerebral Ischemia That May Occur through the Endoplasmic Reticulum Stress Pathway Int J Mol Sci, 2018-06-29;19(7):. 2018-06-29 [PMID: 29966219] (Bioassay, In Vivo, Rat)

Bioinformatics

• Gene Symbol: CDNF
• Uniprot:
  • Q49AH0(Human)