MATN3 Antibody

Western Blot: MATN3 Antibody [NBP1-77022] - Rat thymus tissue lysate with MATN3 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry/Immunofluorescence: MATN3 Antibody [NBP1-77022] - Immunofluorescence of MATN3 in 3T3 cells with MATN3 antibody at 20 ug/mL.

Immunocytochemistry/Immunofluorescence: MATN3 Antibody [NBP1-77022] - 3T3 cells tissue with MATN3 antibody at 2.5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MATN3 Antibody Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human MATN3. The immunogen is located within amino acids 180 - 230 of MATN3. Amino Acid Squence: VTDGRPQDQVNEV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MATN3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 2.5-20 ug/ml
• Control Peptide:

  MATN3 Peptide (NBP1-77022PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MATN3 Antibody

• DIPOA
• EDM5
• HOA
• matrilin 3
• matrilin-3
• OADIP
• OS2

Background

Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). MATN1 and MATN3 are expressed specifically in cartilage and are among the most up-regulated ECM proteins during chondrogenesis. MATN3 is composed of a single N-terminal von Willebrand Factor A (vWFA) domain followed by four epidermal growth factor (EGF) repeats and a coiled-coil domain whereas MATN1 is composed of two vWFA domains separated by one EGF-like domain. MATN1 or MATN3 may play a role in modulating chondrogenesis during the chondrocyte differentiation process. Mutations of this gene have been associated with variety of inherited chondrodysplasias. Recent studies show that aberrant expression and processing of MATN3 are hallmarks of conventional cartilaginous neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MATN3 Antibody (NBP1-77022)(1)

Publication using NBP1-77022

• Ly K, Essalmani R, Desjardins R et al. An Unbiased Mass Spectrometry Approach Identifies Glypican-3 as an Interactor of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR) in Hepatocellular Carcinoma Cells. J Biol Chem 2016 Nov 18 [PMID: 27758865] (Human)

Bioinformatics

• Gene Symbol: MATN3
• Uniprot:
  • AAI39908(Human)