Recombinant Human CD5 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human CD5 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Zymosan is coated at 200 μg/mL (100 μL/well), the concentration of Recombinant Human CD5 that produces 50% optimal binding response is typically 0.6-3 μg/mL.
Mouse myeloma cell line, NS0-derived human CD5 protein
Arg25-Asn371, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Structure / Form
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
39.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
55 kDa, reducing conditions
Read Publication using 1636-CD.

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD5 Protein, CF

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1


CD5, also known as Leu-1, Ly-1, and T1, is a 67 kDa transmembrane glycoprotein in the scavenger receptor superfamily (1). Mature human CD5 consists of a 348 amino acid (aa) extracellular domain (ECD) with three scavenger receptor cysteine-rich (SRCR) domains, a 30 aa transmembrane segment, and a 93 aa cytoplasmic domain (2). Within the ECD, human CD5 shares 55% aa sequence identity with mouse and rat CD5. The 52 kDa ECD can be cleaved from the cell surface and circulates in the serum (3). CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls (4‑6). CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is up‑regulated on tolerized peripheral CD4+ cells (7, 8). It inhibits TCR signaling and promotes T cell nonresponsiveness and survival (8‑10). CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells (11, 12). Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells (13‑16). Similarly to on T cells, it negatively regulates signaling through the B cell antigen receptor and supports peripheral B cell survival, anergy, and tolerance (13, 14, 16). B cells can produce an intracellularly-retained form of CD5 which lacks the signal peptide and a portion of the first SRCR domain (17). CD5 is also involved in the cellular entry of hepatitis C virus into T cells (18).
  1. Soldevila, G. et al. (2011) Curr. Opin. Immunol. 23:310.
  2. Jones, N.H. et al. (1986) Nature 323:346.
  3. Calvo, J. et al. (1999) Tissue Antigens 54:128.
  4. Brown, M.H. and E. Lacey (2010) J. Immunol. 185:6068.
  5. Van de Velde, H. et al. (1991) Nature 351:662.
  6. Vera, J. et al. (2009) Proc. Natl. Acad. Sci. 106:1506.
  7. Azzam, H.S. et al. (1998) J. Exp. Med. 188:2301.
  8. Hawiger, D. et al. (2004) Immunity 20:695.
  9. Tarakhovsky, A. et al. (1995) Science 269:535.
  10. Friedlein, G. et al. (2007) J. Immunol. 178:6821.
  11. Ordonez-Rueda, D. et al. (2009) Eur. J. Immunol. 39:2233.
  12. de Wit, J. et al. (2011) Blood 118:6107.
  13. Bikah, G. et al. (1996) Science 274:1906.
  14. Hippen, K.L. et al. (2000) J. Exp. Med. 191:883.
  15. Yanaba, K. et al. (2008) Immunity 28:639.
  16. Gary-Gouy, H. et al. (2002) Blood 100:4537.
  17. Renaudineau, Y. et al. (2005) Blood 106:2781.
  18. Sarhan, M.A. et al. (2012) J. Virol. Epub.

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Publications for CD5 (1636-CD)(1)

We have publications tested in 1 application: Bioassay.

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Gene Symbol CD5