Recombinant Human CD5 Protein, CF

Images

 
There are currently no images for CD5 (1636-CD).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human CD5 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Zymosan is coated at 200 μg/mL (100 μL/well), the concentration of Recombinant Human CD5 that produces 50% optimal binding response is typically 0.6-3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CD5 protein
Arg25-Asn371, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Arg25
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Gene
CD5
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
39.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55 kDa, reducing conditions
Publications
Read Publication using 1636-CD.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD5 Protein, CF

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Background

CD5, also known as Leu-1, Ly-1, and T1, is a 67 kDa transmembrane glycoprotein in the scavenger receptor superfamily (1). Mature human CD5 consists of a 348 amino acid (aa) extracellular domain (ECD) with three scavenger receptor cysteine-rich (SRCR) domains, a 30 aa transmembrane segment, and a 93 aa cytoplasmic domain (2). Within the ECD, human CD5 shares 55% aa sequence identity with mouse and rat CD5. The 52 kDa ECD can be cleaved from the cell surface and circulates in the serum (3). CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls (4‑6). CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is up‑regulated on tolerized peripheral CD4+ cells (7, 8). It inhibits TCR signaling and promotes T cell nonresponsiveness and survival (8‑10). CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells (11, 12). Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells (13‑16). Similarly to on T cells, it negatively regulates signaling through the B cell antigen receptor and supports peripheral B cell survival, anergy, and tolerance (13, 14, 16). B cells can produce an intracellularly-retained form of CD5 which lacks the signal peptide and a portion of the first SRCR domain (17). CD5 is also involved in the cellular entry of hepatitis C virus into T cells (18).
  1. Soldevila, G. et al. (2011) Curr. Opin. Immunol. 23:310.
  2. Jones, N.H. et al. (1986) Nature 323:346.
  3. Calvo, J. et al. (1999) Tissue Antigens 54:128.
  4. Brown, M.H. and E. Lacey (2010) J. Immunol. 185:6068.
  5. Van de Velde, H. et al. (1991) Nature 351:662.
  6. Vera, J. et al. (2009) Proc. Natl. Acad. Sci. 106:1506.
  7. Azzam, H.S. et al. (1998) J. Exp. Med. 188:2301.
  8. Hawiger, D. et al. (2004) Immunity 20:695.
  9. Tarakhovsky, A. et al. (1995) Science 269:535.
  10. Friedlein, G. et al. (2007) J. Immunol. 178:6821.
  11. Ordonez-Rueda, D. et al. (2009) Eur. J. Immunol. 39:2233.
  12. de Wit, J. et al. (2011) Blood 118:6107.
  13. Bikah, G. et al. (1996) Science 274:1906.
  14. Hippen, K.L. et al. (2000) J. Exp. Med. 191:883.
  15. Yanaba, K. et al. (2008) Immunity 28:639.
  16. Gary-Gouy, H. et al. (2002) Blood 100:4537.
  17. Renaudineau, Y. et al. (2005) Blood 106:2781.
  18. Sarhan, M.A. et al. (2012) J. Virol. Epub.

Customers Who Viewed This Item Also Viewed...

NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-43435
Species: Hu, Mu
Applications: WB, Flow
NBP1-85599
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, In vitro, CyTOF-ready
NBP2-25200
Species: Hu
Applications: WB, Flow, IHC, IHC-Fr, B/N
AF1182
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
AF7579
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NB100-77417
Species: Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
AF123
Species: Hu
Applications: WB, Block, ICC
NBP2-24695
Species: Hu
Applications: WB, IHC-P
6507-IL/CF
Species: Hu
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF

Publications for CD5 (1636-CD)(1)

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
All Species

Reviews for CD5 (1636-CD) (0)

There are no reviews for CD5 (1636-CD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CD5 (1636-CD) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for CD5 (1636-CD)

Discover related pathways, diseases and genes to CD5 (1636-CD). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD5 (1636-CD)

Discover more about diseases related to CD5 (1636-CD).
 

Pathways for CD5 (1636-CD)

View related products by pathway.

PTMs for CD5 (1636-CD)

Learn more about PTMs related to CD5 (1636-CD).

Blogs on CD5

There are no specific blogs for CD5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4
6507-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CD5 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CD5
Uniprot