Recombinant Human CD277/BTN3A1 Fc Chimera Protein, CF

Recombinant Human CD277/BTN3A inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-5 µg/mL.

1 μg/lane of RecombinantHuman CD277/BTN3A1 (Catalog # 8539-BT) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing bands at 58.8 and 150 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD277/BTN3A1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is typically 1-5 µg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human CD277/BTN3A1 protein
  

  Human CD277/BTN3A1 (Phe31-Gly254) Accession # O00481	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O00481
• N-terminal Sequence: Phe31
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BTN3A1
• Purity: >90%, by SDS-PAGE with silver staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD277/BTN3A1 Fc Chimera Protein, CF

• BT3.1
• BTF5
• BTN3A1
• butyrophilin, subfamily 3, member A1
• CD277
• dJ45P21.3 (butyrophilin, subfamily 3, member A1)

Background

Butyrophilin 3A1 (also called BTN3A1) is a 57 kDa type I transmembrane glycoprotein member of the Ig superfamily. It is expressed on a wide variety of immune cells. Similar to BTN3A2 and BTN3A3, BTN3A1 (484 amino acids) is composed of an extracellular N-terminal IgV and a membrane-proximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. These Ig domains are also found in B7 family co-stimulatory molecules, suggesting structural and functional similarities between the two protein families (1). The intracellular portion of BTN3A1 contains a B30.2 domain (2). Although the B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) and is conserved among BTN1A1 orthologs, this interaction with XOR is not shared by BTN3A1 (3). The B30.2 domain of butyrophilins also functions as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations produced during tumorigenesis and microbial infections (4, 5). The specific binding of pAg by the B30.2 domain of BTN3A1 induces a conformational change in its ECD, leading to the activation of V gamma 9Vδ2 T cells (6). Thus, BTN3A1 acts as a critical protein for the activation of V gamma 9Vδ2 T cells following detection of distressed cells (7). The anti-tumor responses of V gamma 9Vδ2 T cells may be enhanced with agonistic anti-BTNA3 antibodies (8). No BTN3A1 homolog has yet been identified in rodents. However, Human BTN3A1 shares 92.4% and 91.6% sequence identity with baboon and rhesus monkey BTN3A1 respectively (9).

1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
2. Rhodes, D.A. et al. (2001) Genomics 71:351.
3. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
4. Harly, C. et al. (2012) Blood 120:2269.
5. Constant, P. et al. (1994) Science 264:267.
6. Sandstrom, A. et al. (2014) Immunity 40:490.
7. Harly, C. et al. (2015) Front. Immunol. 5:657.
8. Bonneville, M. et al. (2006) Curr. Opin. Immunol. 18:539.
9. Wang, H. et al. (2013) J.Immunol. 191:1029.

Bioinformatics

• Gene Symbol: BTN3A1
• Uniprot:
  • O00481(Human)