Recombinant Human CD109 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human CD109 Protein, CF Summary

Details of Functionality
Measured by its ability to bind TGF-beta 1 in a functional ELISA. Finnson, K.W. et al. (2006) FASEB J. 20:1525. Immobilized rhTGF-beta 1 at 1 µg/mL (100 µL/well) can bind rhCD109 with an apparent KD <0.1 µM.
Source
Mouse myeloma cell line, NS0-derived human CD109 protein
Val22-Ser1268 (Tyr703Ser & Thr1241Met), with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Val22
Protein/Peptide Type
Recombinant Proteins
Gene
CD109
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
155-165 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD109 Protein, CF

  • 150 kDa TGF-beta-1-binding protein
  • activated T-cell marker CD109
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7
  • CD109 antigen (Gov platelet alloantigens)
  • CD109 antigen
  • CD109 molecule
  • CD109
  • CPAMD7
  • CPAMD7r150
  • DKFZp762L1111
  • FLJ38569
  • FLJ41966
  • Gov platelet alloantigens
  • p180
  • Platelet-specific Gov antigen
  • RP11-525G3.1

Background

CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares71% - 73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27% - 33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5 - 9). It is produced as a 170 kDa - 180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

  1. Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
  2. Lin, M. et al. (2002) Blood 99:1683.
  3. Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
  4. Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
  5. Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
  6. Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
  7. Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
  8. Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
  9. Hashimoto, M. et al. (2004) Oncogene 23:3716.
  10. Solomon, K.R. et al. (2004) Gene 327:171.
  11. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
  12. Schuh, A.C. et al. (2002) Blood 99:1692.
  13. Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
  14. Rozman, P. (2002) Transpl. Immunol. 10:165.

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Bioinformatics

Gene Symbol CD109
Uniprot