Recombinant Human CCL7/MCP-3 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CCL7/MCP-3 Protein Summary

Details of Functionality
Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485. The ED50 for this effect is 0.02-0.08 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 0.1-0.5 µg/mL.
Source
E. coli-derived human CCL7/MCP-3/MARC protein
Gln34-Leu109
Accession #
N-terminal Sequence
Gln34
Protein/Peptide Type
Recombinant Proteins
Gene
CCL7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
282-P3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL7/MCP-3 Protein

  • C-C motif chemokine 7
  • CCL7
  • chemokine (C-C motif) ligand 7
  • FIC
  • MARC
  • MCP-3
  • MCP3Monocyte chemotactic protein 3
  • MCP-3Small-inducible cytokine A7
  • MGC138465
  • Monocyte chemoattractant protein 3
  • NC28SCYA6
  • SCYA7MGC138463
  • small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

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Publications for CCL7/MCP-3/MARC (282-P3)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(7)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 282-P3 Applications Species
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology, 2010;129(4):536-46. 2010 [PMID: 20002784] (Bioassay, Human) Bioassay Human
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010;127(2):332-44. 2010 [PMID: 19937793] (Bioassay, Human) Bioassay Human
Nguyen AN, Stebbins EG, Henson M, O&amp;amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation. Exp. Cell Res., 2006;312(10):1909-23. 2006 [PMID: 16600214] (Bioassay, Human) Bioassay Human
Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005;94(2):292-300. 2005 [PMID: 15765748] (Bioassay, Human) Bioassay Human
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004;173(11):6592-602. 2004 [PMID: 15557149] (Bioassay, Human) Bioassay Human
Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002;456(1):1-10. 2002 [PMID: 12450563] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol CCL7
Uniprot