Recombinant Human C1qTNF10/C1qL2 Protein, CF

When Recombinant Human C1qTNF10 (Catalog #9189-TN)is coated at 2 μg/mL, 100 μL/well, Recombinant Human BAI2 Fc Chimera binds with a ED50 of0.075-0.45 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human C1qTNF10/C1qL2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human C1qTNF10/C1qL2 is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Protein Fc Chimera (Catalog # 9338-BA) that produces 50% of the optimal binding response is approximately 0.075-0.45 μg/mL.
• Source: E. coli-derived human C1qTNF10/C1qL2 protein
  Phe154-Asp287, with an N-terminal Met and 6-His tag
• Accession #: Q7Z5L3
• N-terminal Sequence: Met
• Structure / Form: Noncovalently-linked homotrimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 14 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP and Trehalose.
• Purity: >70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human C1qTNF10/C1qL2 Protein, CF

• C1q and tumor necrosis factor related protein 10
• C1q-domain containing protein
• C1qL2
• C1qTNF10
• complement C1q-like protein 2
• complement component 1, q subcomponent-like 2
• CTRP10

Background

C1qTNF10 (CTRP10), also known as C1qL2, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF10 contains a collagen-like region and one C1q-like domain. It can form heterotrimers and higher order disulfide linked oligomers with C1qTNF1, C1qTNF11/C1qL4, and C1qTNF13/C1qL3 (3-6). Within the C1q-like domain, human C1qTNF10 shares 100% aa sequence identity with mouse and rat C1qTNF10. Similarly to C1qTNF11, C1qTNF13, and C1qTNF14/C1qL1, C1qTNF10 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (7). It is produced by mossy fibers in the hippocampus where it enhances the postsynaptic clustering of kainate-type glutamate receptors (KAR) on CA3 pyramidal neurons (8). This function is enhanced by the binding of C1qTNF10 to the KAR subunits GluK2 and GluK4 as well as to Neurexin-3 beta containing the exon 5b splice site insertion (8).

1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
2. Yuzaki, M. (2001) Eur. J. Neurosci. 32:191.
3. Peterson, J.M. et al. (2009) Biochem. Biophys. Res. Commun. 388:360.
4. Wei, Z. et al. (2011) J. Biol. Chem. 286:15652.
5. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
6. Wong, G.W. et al. (2008) Biochem. J. 416:161.
7. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
8. Matsuda, K. et al. (2016) Neuron 90:752.

Bioinformatics

• Uniprot:
  • Q7Z5L3()