Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

When Recombinant Human BTN2A1/Butyrophilin 2A1 (Catalog # 9058-BT) is coated at 1 µg/mL, Recombinant Human DC-SIGN/CD209 Fc Chimera (Catalog # 161-DC) binds with a ED50 of0.1-0.5 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human BTN2A1 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 161-DC) that produces 50% of the optimal binding response is approximately
  0.1-0.5 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human BTN2A1 protein
  Gln29-Ala248, with a C-terminal 6-His tag
• Accession #: Q7KYR7
• N-terminal Sequence: No results obtained. Gln29 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: BTN2A1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF

• BK14H9.1
• BT2.1
• BTF1
• BTF1BT2.1butyrophilin BTF1
• BTN2A1
• butyrophilin subfamily 2 member A1
• butyrophilin, subfamily 2, member A1
• DJ3E1.1
• FLJ36567

Background

Butyrophilin 2A1 (BTN2A1) is an approximately 70 kDa widely expressed and variably glycosylated type I transmembrane glycoprotein (1). Mature human Butyrophilin 2A1 consisits of a 220 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 258 aa cytoplasmic domain (2). Alternative splicing generates additional isoforms of human Butyrophilin 2A1 that lack the first Ig-like domain or transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (3, 4). It binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (3). The polymorphism of BTN2A1 has been associated with metabolic syndrome, type II diabetes mellitus, chronic kidney disease, and hypertension (5, 6).

1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
2. Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
3. Malcherek, G. et al. (2007) J. Immunol. 179:3804.
4. Cavaletto, M. et al. (2002) Proteomics 2:850.
5. Oguri, M. et al.(2011) J. Med. Genet. 48:787.
6. Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.

Publications for BTN2A1 (9058-BT)(1)

Publication using 9058-BT

• CE Cano, C Pasero, A De Gassart, C Kerneur, M Gabriac, M Fullana, E Granarolo, R Hoet, E Scotet, C Rafia, T Hermman, C Imbert, L Gorvel, N Vey, A Briantais, AC le Floch, D Olive BTN2A1, an immune checkpoint targeting Vgamma9Vdelta2 T cell cytotoxicity against malignant cells Cell Reports, 2021-07-13;36(2):109359. 2021-07-13 [PMID: 34260935] (ELISA Capture, Immunization, Human, Mouse)

Bioinformatics

• Gene Symbol: BTN2A1
• Uniprot:
  • Q7KYR7()