Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein Summary

Additional Information
A New rh BMPR-IA is Available! ~2 fold better activity; Lower endotoxin specification; Enhanced purity!
Details of Functionality
Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1‑0.3 µg/mL in the presence of 30 ng/mL of recombinant human BMP-4.
Source
Mouse myeloma cell line, NS0-derived human BMPR-IA/ALK-3 protein
Human BMPR-IA
(Gln24 - Arg152)
Accession # CAA80257
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMPR1A
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
41.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55 kDa, reducing conditions
Publications
Read Publications using
315-BR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

  • ACVRLK310q23del
  • ALK-3
  • ALK3EC 2.7.11.30
  • BMP type-1A receptor
  • BMPR1A
  • BMPR-1A
  • BMPRIA
  • BMPR-IA
  • bone morphogenetic protein receptor type-1A
  • bone morphogenetic protein receptor, type IA
  • CD292 antigen
  • CD292
  • EC 2.7.11
  • Serine/threonine-protein kinase receptor R5
  • SKR5
  • type II-like kinase 3

Background

Bone Morphogenetic Protein Receptor IA (BMPR-IA), also known as ALK-3, BRK-1, and CD292, is a glycosylated 60 - 65 kDa type I receptor in the TGF‑ beta serine/threonine kinase receptor family (1 ‑ 3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Mature human BMPR-IA consisits of a 129 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane segment, and a 356 aa cytoplasmic region that contains the tyrosine kinase domain (4). Within the ECD, human BMPR-IA shares 98% and 97% aa sequence identity with mouse and rat BMPR-IA, respectively. BMPR-IA is involved in the development and function of a wide range of tissues. During early embryogenesis it is required for migration of the anterior visceral endoderm (AVE) and proper development of the anterior-posterior axis (5). Tissue-specific conditional knockout experiments have demonstrated the importance of BMPR-IA in the development and morphogenesis of the heart, lung, palate, teeth, and mandible (6 - 8). In the adult, BMPR-IA plays a role in glucose-stimulated insulin secretion by pancreatic beta cells, osteoclast activity and bone remodeling, reactive astrocyte-mediated scar formation following spinal cord injury, and ovulation and fertility (9 - 12).
  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  5. Miura, S. et al. (2010) Dev. Biol. 341:246.
  6. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
  7. Sun, J. et al. (2008) Am. J. Pathol. 172:571.
  8. Li, L. et al. (2011) Dev. Biol. 349:451.
  9. Goulley, J. et al. (2007) Cell Metab. 5:207.
  10. Kamiya, N. et al. (2008) J. Bone Miner. Res. 23:2007.
  11. Sahni, V. et al. (2010) J. Neurosci. 30:1839.
  12. Edson, M.A. et al. (2010) Mol. Endocrinol. 24:1251.

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315-BR
Species: Hu
Applications: Bioactivity

Publications for BMPR-IA/ALK-3 (315-BR)(11)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 3 applications: Bioassay, Receptor Binding Assay, Surface Plasmon Resonance.


Filter By Application
Bioassay
(7)
Receptor Binding Assay
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(9)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 315-BR Applications Species
T Mang, K Kleinschmi, F Ploeger, A Schoeneman, S Lindemann, A Gigout BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation J. Cell. Sci., 2020;0(0):. 2020 [PMID: 32764110] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. PLoS ONE, 2015;10(6):e0131453. 2015 [PMID: 26110906] (Bioassay, Human) Bioassay Human
Woltje K, Jabs M, Fischer A Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells. PLoS ONE, 2015;10(3):e0120547. 2015 [PMID: 25799559] (Bioassay, Human) Bioassay Human
Bigley V, McGovern N, Milne P, Dickinson R, Pagan S, Cookson S, Haniffa M, Collin M Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells. J Leukoc Biol, 0;97(4):627-34. 0 [PMID: 25516751] (Bioassay, Human) Bioassay Human
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Steinbicker AU, Sachidanandan C, Vonner AJ Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation. Blood, 2011;117(18):4915-23. 2011 [PMID: 21393479] (Bioassay, Human) Bioassay Human
Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood, 2008;112(8):3154-63. 2008 [PMID: 18664625] (Bioassay, Human) Bioassay Human
Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008;381(4):1025-39. 2008 [PMID: 18621057]
David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells. Blood, 2007;109(5):1953-61. 2007 [PMID: 17068149] (Bioassay, Human) Bioassay Human
Reversade B, De Robertis EM Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field. Cell, 2005;123(6):1147-60. 2005 [PMID: 16360041] (Receptor Binding Assay, Human) Receptor Binding Assay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol BMPR1A
Uniprot