Recombinant Human Attractin Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Attractin Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When recombinant mouse Agouti is coated at 1 μg/mL, Recombinant Human Attractin binds with apparent K_D <40 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Attractin protein
  

  Human Attractin (Ala84-Gln1272) Accession # NP_647538	HPGGGSGGGSGGGS	6-His tag
  N-terminus		C-terminus

• Accession #: NP_647538
• N-terminal Sequence: Ala84
• Protein/Peptide Type: Recombinant Proteins
• Gene: ATRN
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150-180 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Attractin Protein, CF

• ATRN
• Attractin
• DPPT-L
• KIAA0548
• Mahogany Protein
• MGC126754
• MGCA

Background

Attractin, also known as DPPT-L and Mahogany, is a transmembrane glycoprotein with functions in hair pigmentation, axon myelination, energy balance regulation, and immune activation (1, 2). Mature human Attractin consists of a 1196 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 129 aa cytoplasmic domain (3). Within the ECD, it shares 95% aa sequence identity with mouse and rat Attractin. Alternative splicing generates an approximately 175 kDa secreted soluble isoform that corresponds to the ECD of the transmembrane form (4, 5). Attractin is expressed in activated T cells, monocytes, hair follicle melanocytes, brain (notably the hypothalamus, hippocampus, and substantia nigra), and adipose tissue (3, 6-9). Activated T cells release soluble Attractin which then co-stimulates T cell proliferation (3, 4). Attractin also functions with Melanocortin 1R as a co-receptor for Agouti and, as a soluble molecule, can neutralize the bioactivity of Agouti on hair follicle melanocytes (7, 8, 10, 11). Similarly, mutations in Attractin gene suppresses the diet-induced obesity which is characteristic of Agouti overexpressing mice (1, 7). In the CNS, Attractin plays a role in axon myelination, neurite branching on differentiating neurons, and protection from neurotoxins (1, 9, 12). It is elevated in the CSF in high grade malignant astrocytoma and promotes glioma cell migration (14).

1. Gunn, T.M. et al. (2001) Genetics 158:1683.
2. Friedrich, D. et al. (2007) Biol. Chem. 388:155.
3. Duke-Cohan, J.S. et al. (1998) Proc. Natl. Acad. Sci. USA 95:11336.
4. Duke-Cohan, J.S. et al. (1996) J. Immunol. 156:1714.
5. Tang, W. et al. (2000) Proc. Natl. Acad. Sci. USA 97:6025.
6. Wrenger, S. et al. (2006) J. Leukoc. Biol. 80:621.
7. Nagle, D.L. et al. (1999) Nature 398:148.
8. He, L. et al. (2001) Nat. Genet. 27:40.
9. Paz, J. et al. (2007) Neurobiol. Aging 28:1446.
10. Hida, T. et al. (2009) Pigment Cell Melanoma Res. 22:623.
11. Gunn, T.M. et al. (1999) Nature 398:152.
12. Kuramoto, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:559.
13. Tang, W. and J.S. Duke-Cohan (2002) J. Neuropathol. Exp. Neurol. 61:767.
14. Khwaja, F.W. et al. (2006) Clin. Cancer Res. 12:6331.

Bioinformatics

• Gene Symbol: ATRN
• Uniprot:
  • NP_647538()