Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human BMP-6 (Catalog # 507-BP) is used at 0.25 μg/mL, the concentration of Recombinant Human Activin RIA/ALK-2 Fc Chimera that produces 50% of the optimal binding response is found to be approximately
1-5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Activin RIA/ALK-2 protein
Human Activin RIA
(Asp23-Val124 & Phe120-Val124)
Accession # Q53SV1
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp23 & Phe120
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
ACVR1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
39 kDa & 28 kDa (monomers).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-45 kDa & 34-38 kDa, reducing conditions
Publications
Read Publications using
637-AR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

  • activin A receptor, type I
  • Activin RIA
  • ActivinRIA
  • ACTRI
  • ACVR1
  • ACVR1A
  • ACVRLK2
  • ALK2
  • ALK-2
  • FOP
  • SKR1
  • TSRI

Background

Activin RIA, also known as ALK-2, TSK-7L, SKR1, TSR-I, and ACTR-I, is a glycosylated 65 kDa type I receptor in the TGF-beta serine/threonine kinase receptor family (1-3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis
(1, 4-7). Mature human Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Ser-rich GS box and the kinase domain (8, 9). Within the ECD, human Activin RIA shares 99% and 94% aa sequence identity with mouse and rat Activin RIA, respectively. It shares 20%-26% aa sequence identity with other human type I receptors Activin RIB, BMPR-IA, BMPR-IB, and TGF-beta RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGM-B/DRAGON, a BMP coreceptor that also associates with other type I and type II receptors (10). Activin RIA can additionally phosphorylate the coreceptor Endoglin and is required for the inhibitory effect of Endoglin on prostate cancer cell motility (11).
  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. Chuva de Sousa Lopes, S.M. et al. (2004) Genes Dev. 18:1838.
  5. Zhang, D. et al. (2003) J. Bone Miner. Res. 18:1593.
  6. van Dinther, M. et al. (2010) J. Bone Miner. Res. 25:1208.
  7. Wang, J. et al. (2005) Dev. Biol. 286:299.
  8. Matsuzaki, K. et al. (1993) J. Biol. Chem. 268:12719.
  9. Attisano, L. et al. (1993) Cell 75:671.
  10. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  11. Romero, D. et al. (2010) Carcinogenesis 31:359.

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Applications: Binding Activity

Publications for Activin RIA/ALK-2/Activin Receptor Type 1 (637-AR)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, ELISA Detection, Receptor Binding Assay.


Filter By Application
Bioassay
(1)
ELISA Detection
(1)
Receptor Binding Assay
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
All Species

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Bioinformatics

Gene Symbol ACVR1
Entrez
Uniprot