Recombinant Feline Flt-3 Ligand/FLT3L Protein

• Reactivity: Fe
• Applications: Bioactivity

Recombinant Feline Flt-3 Ligand/FLT3L Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED₅₀ for this effect is 0.100-1.00 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived feline Flt-3 Ligand/FLT3L protein
  Ser27-Pro185, with a C-terminal 6-His tag
• Accession #: NP_001009842
• N-terminal Sequence: Ser27
• Protein/Peptide Type: Recombinant Proteins
• Gene: FLT3LG
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-33 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Feline Flt-3 Ligand/FLT3L Protein

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FLT3L, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature feline Flt-3 Ligand consists of a 156 amino acid (aa) extracellular region with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and an 88 aa cytoplasmic tail (4, 5). Within the ECD, feline Flt-3 Ligand shares 92%, 81%, 70%, and 63% aa sequence identity with canine, human, mouse, and rat Flt-3 Ligand, respectively. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 6). Each 36 kDa chain of the Flt-3 Ligand dimer carries approximately 12 kDa of N- and O-linked carbohydrates (6). Alternate splicing and proteolytic cleavage of the transmembrane form of the Flt-3 Ligand protein can generate a soluble 30 kDa fragment that includes the cytokine-like domain (6, 7). Multiple splice forms of canine, human, and mouse Flt-3 Ligand have been described and may also exist in feline (4, 6-10). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 5, 7, 10). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 11). Additionally, Flt-3 Ligand synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (7, 8, 10). Flt-3 Ligand also cooperates with IL-2, IL-6, IL-7, and -15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation (1, 12). Animal studies also show Flt-3 Ligand reduces the severity of experimentally induced allergic inflammation (13).

1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
4. Yang, S. and G.-K. Sim (2000) DNA Seq. 11:163.
5. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
6. McClanahan, T. et al. (1996) Blood 88:3371.
7. Hannum, C. et al. (1994) Nature 368:643.
8. Lyman, S.D. et al. (1994) Blood 83:2795.
9. Lyman, S.D. et al. (1995) Oncogene 10:149.
10. Lyman, S.D. et al. (1993) Cell 75:1157.
11. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
12. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
13. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

Bioinformatics

• Gene Symbol: FLT3LG
• Uniprot:
  • NP_001009842()