Recombinant Cynomolgus SIRP gamma/CD172g Fc Protein, CF

Recombinant Cynomolgus Monkey SIRPgamma/CD172g Fc Chimera (Catalog # 10265-SG) inhibits anti-CD3-induced proliferation of human T cells. The ED50 for this effect 1‑8 μg/mL.

2 μg/lane of Recombinant Cynomolgus Monkey SIRP gamma /CD172g Fc Chimera (Catalog # 10265-SG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 82-90 kDa and 170-190 kDa, respectively.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus SIRP gamma/CD172g Fc Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 1-8 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey SIRP gamma/CD172g protein
  

  Cynomolgus Monkey SIRP gamma /CD172g (Glu29-His360) Accession # XP_005568591.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: XP_005568591.1
• N-terminal Sequence: Glu29
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 82-90 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus SIRP gamma/CD172g Fc Protein, CF

• bA77C3.1
• CD172g antigen
• CD172g
• FLJ42230
• signal-regulatory protein beta 2
• Signal-regulatory protein beta-2
• signal-regulatory protein gamma
• SIRP beta 2
• SIRP gamma
• SIRP-B2
• SIRPB2CD172 antigen-like family member B
• SIRP-beta-2
• SIRPG
• SIRPgamma
• SIRP-gamma

Background

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are "paired receptors" with homology in the extracellular domain but variability in the C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, cynomolgus monkey SIRP gamma isoform 1 shares 91% aa identity with human SIRP gamma (2).SIRP gamma is the only SIRP known to be expressed on T cells, CD56^bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It binds to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling motif (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5). Engagement of CD47 by SIRP gamma was shown to induce apoptosis on T cell and monocyte cell lines (6).

1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
2. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
5. Piccio, L. et al. (2005) Blood 105:2421.
6. Brooke, G. et al. (2004) J. Immunol. 173:2562.

Bioinformatics

• Uniprot:
  • XP_005568591.1()