Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.75‑6.0 ng/mL.

2 μg/lane of Recombinant Cynomolgus Monkey IL-27 His-tag (Catalog # 10307-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-65 kDa.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The ED₅₀ for this effect is 0.75-6.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-27 protein
  

  Cynomolgus Monkey EBI-3 (Arg36-Lys244) Accession # XP_005587614.1	GSGSSRGGSGSGGSGGGGSKL	Cynomolgus Monkey IL-27a *Unique sequence	6-His tag
  N-terminus			C-terminus

  
  * Unique Sequence: This gene was isolated from a cynomolgus monkey cDNA library. The closest match to the obtained sequence is a genomic prediction for rhesus monkey, Accession XP_028697194.1 (Phe42-Pro254, A170T).
• Accession #: XP_005587614.1 (EBI-3) & XP_028697194.1 (IL-27a)
• N-terminal Sequence: Arg36
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-65 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF

• IL-27 p28 subunit
• IL-27 subunit alpha
• IL27
• IL-27
• IL27A
• IL-27-A
• IL27-A
• IL27p28
• IL30
• interleukin 27
• interleukin 30
• interleukin-27 subunit alpha
• MGC71873
• p28IL-27A

Background

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 50 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, a 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The Cynomolgous EBI3 gene encodes a 244 amino acid (aa) precursor that contains a 36 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Cynomolgous monkey EBI3 is 94% aa identical to Human EBI3. The Cynomolgous monkey p28 gene encodes a 254 aa precursor that contains a 41 aa signal sequence and 214 aa mature regions. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Cynomolgous monkey p28 is 92% aa identical to human p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory cytokine, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

1. Boulay, J-L. et al. (2003) Immunity 19:159.
2. Trinchieri, G. et al. (2003) Immunity 19:641.
3. Murakami, M. et al. (2004) Growth Factors 22:75.
4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715.
5. Devergne, O. et al. (1996) J. Virology 70:1143.
6. Pflanz, S. et al. (2002) Immunity 16:779.
7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
8. Pflanz, S. et al. (2004) J Immunol 172:2225.
9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
10. Holscher, C. (2004) Med. Microbiol. Immunol. 193:1.

Bioinformatics

• Uniprot:
  • XP_005587614.1 (EBI-3) & XP_028697194.1 (IL-27a)()