Recombinant Cotton Rat GRO Protein

• Reactivity: Cr
• Applications: Bioactivity

Recombinant Cotton Rat GRO Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 0.4-2 ng/mL.
• Source: E. coli-derived cotton rat CXCL1/2/3/GRO protein
  Ala28-Lys100
• Accession #: AAL16934
• N-terminal Sequence: Ala28
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 7.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cotton Rat GRO Protein

• CXCL1/2/3
• FSP
• GRO1
• GROa
• MGSA
• MGSA-a
• NAP-3
• SCYB1

Background

Cotton rat growth-regulated protein (GRO) is a member of the ELR⁺ CXC subfamily of chemokines (1). The cotton rat GRO cDNA encodes a 100 amino acid (aa) residue precursor protein with a 27 aa putative signal peptide and a 73 aa mature protein (2). Among the three human GRO proteins, mature cotton rat GRO is most closely related to GRO alpha /CXCL1, sharing approximately 77% aa sequence identity (3). It also shares 75% and 70% aa sequence identity with mature human GRO beta /CXCL2 and GRO gamma /CXCL3, respectively (4). Compared with rat CXC chemokines, mature cotton rat shows 92%, 76%, and 76% aa sequence homology with rat KC/GRO/CINC-1, CINC-2 and CINC-3, respectively (5). Mature cotton rat GRO also shares 74% sequence homology with mature cotton rat MIP-2 (6). Similarly to GRO proteins from other species, cotton rat GRO is expected to be a potent neutrophil chemoattractant and activator. Cotton rat GRO has been shown to bind and activate both mouse chemokine receptors CXCR1 and CXCR2 (7). Similarly to human GRO alpha , the cotton rat GRO concentration required to activate CXCR1 is at least an order of magnitude higher than that required to activate CXCR2 (7, 8). Based on studies on rodents, cotton rat GRO is predicted to be produced by a wide variety of cell types including macrophages, endothelial cells, fibroblasts, astrocytes, neutrophils, keratinocytes and intestinal epithelium (1, 9).

1. Rollins, B.J. (1997) Blood 90:909.
2. Blanco, J.C. et al. (2001) GenBank Accession #: AAL16934.
3. Richmond, A. et al. (1988) EMBO J. 7:2025.
4. Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
5. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
6. Blanco, J.C. et al. (2001) GenBank Accession #: AAL26705.
7. R&D Systems, unpublished observations.
8. Schumacher, C. et al. (1992) Proc. Natl. Acad. Sci. USA 89:10542.
9. Zlotnik, A.O. (2000) Immunity 12:121.

Bioinformatics

• Uniprot:
  • AAL16934()