Recombinant Chicken Caronte Fc Chimera Protein, CF

• Reactivity: Ch
• Applications: Binding Activity, Bioactivity
• Format: Carrier-Free

Recombinant Chicken Caronte Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to cause heart reversals when injected into the right lateral plate of a population of Xenopus neurulae embryos. 80 ng per embryo induces 25-50% heart reversals. Measured by its binding ability in a functional ELISA. Immobilized rchCaronte/Fc Chimera at 5 µg/mL (100 µL/well) can bind hBMP-4 with a linear range of 0.8-100 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived chicken Caronte protein
  

  Chicken Caronte (Asp16 - Asn272) and (Glu18 - Asn272) Accession # NP_990154	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_990154
• N-terminal Sequence: Asp16 & Glu18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CER1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• Theoretical MW: 56 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Chicken Caronte Fc Chimera Protein, CF

• Caronte
• Chicken Caronte

Background

Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.

1. Pearce, J. et al. (1999) Dev. Biol. 209:98.
2. Yokouchi, Y. et al. (1999) Cell. 98:573.
3. King, T. and N. Brown (1999) Nature 401:222.
4. Ahu, L. et al. (1999) Curr. Biol. 9:931.
5. Rodriguez-Esteban, C. et al. (1999) Nature 401:243.

Bioinformatics

• Gene Symbol: CER1
• Uniprot:
  • NP_990154()