RDH14 Antibody (OTI1A7) - Azide and BSA Free

Western Blot: RDH14 Antibody (OTI1A7) - Azide and BSA Free [NBP2-73847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RDH14 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RDH14.

Flow Cytometry: RDH14 Antibody (OTI1A7) - Azide and BSA Free [NBP2-73847] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RDH14 antibody, and then analyzed by flow cytometry.

Western Blot: RDH14 Antibody (OTI1A7) - Azide and BSA Free [NBP2-73847] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RDH14 monoclonal antibody.

Flow Cytometry: RDH14 Antibody (OTI1A7) - Azide and BSA Free [NBP2-73847] - Analysis of Hela cells, using anti-RDH14 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: RDH14 Antibody (OTI1A7) - Azide and BSA Free [NBP2-73847] - Analysis of Jurkat cells, using anti-RDH14 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, CyTOF-ready
• Clone: OTI1A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RDH14 Antibody (OTI1A7) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human RDH14 (NP_065956) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RDH14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Western Blot 1:200-500
• Theoretical MW: 36.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RDH14 Antibody (OTI1A7) - Azide and BSA Free

• Alcohol dehydrogenase PAN2
• EC 1.1.1.-
• PAN2retinol dehydrogenase 14
• pancreas protein 2
• retinol dehydrogenase 14 (all-trans and 9-cis)
• retinol dehydrogenase 14 (all-trans/9-cis/11-cis)
• SDR7C4
• short chain dehydrogenase/reductase family 7C, member 4

Background

Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinalreductase. Displays high activity toward 9-cis and all-trans-retinol. No steroid dehydrogenase activity detected

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RDH14