RBX1 Antibody

Western Blot: RBX1 Antibody [NBP2-75643] - Analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: RBX1 Antibody [NBP2-75643] - Staining RBX1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RBX1 Antibody [NBP2-75643] - Analysis of paraffin-embedded mouse colon tissue using anti-RBX1 antibody. Counter stained with hematoxylin.

Flow Cytometry: RBX1 Antibody [NBP2-75643] - Analysis of MCF-7 cells with RBX1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: RBX1 Antibody [NBP2-75643] - Staining RBX1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RBX1 Antibody [NBP2-75643] - Analysis of paraffin-embedded human stomach cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RBX1 Antibody [NBP2-75643] - Analysis of paraffin-embedded human placenta tissue using anti-RBX1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RBX1 Antibody [NBP2-75643] - Analysis of paraffin-embedded human colon cancer tissue using anti-RBX1 antibody. Counter stained with hematoxylin.

Product Discontinued

RBX1 Antibody Summary

• Immunogen: Recombinant protein within Human RBX1 aa 1-108 / 108. (SwissProt: P62877 Human; SwissProt: P62878 Mouse)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBX1 Antibody

• BA554C12.1
• EC 6.3.2.-
• FLJ60363
• MGC13357
• Protein ZYP
• RBX1
• Regulator of cullins 1
• Ring Box 1
• RING box protein 1
• RING finger protein 75
• ring-box 1
• ring-box 1, E3 ubiquitin protein ligase
• RING-box protein 1
• RNF75
• RNF75MGC1481
• ROC1E3 ubiquitin-protein ligase RBX1
• ZYP Protein

Background

ROC1 is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBX1