RBM8A Antibody (PCRP-RBM8A-1B4)

Immunocytochemistry/Immunofluorescence: RBM8A Antibody (PCRP-RBM8A-1B4) [NBP3-07479] - Immunofluorescence Analysis of PFA-fixed HeLa cells using RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: RBM8A Antibody (PCRP-RBM8A-1B4) [NBP3-07479] - Flow Cytometric Analysis of PFA-fixed HeLa cells. RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: RBM8A Antibody (PCRP-RBM8A-1B4) [NBP3-07479] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, MA
• Clone: PCRP-RBM8A-1B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RBM8A Antibody (PCRP-RBM8A-1B4) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08800)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human RBM8A protein (Uniprot: Q9Y5S9)
• Localization: Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein.
• Specificity: RBM8A Antibody is a high quality monoclonal RBM8A antibody (also designated RBM8A antibody) suitable for the detection of the RBM8A protein of mouse, rat, human and Xenopus laevis origin. RBM8A Antibody (4C4) is available as both the non-conjugated anti-RBM8A antibody form, as well as multiple conjugated forms of anti-RBM8A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include RBM8A, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and RBM8A, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, RBM8A, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human RBM8A encodes three transcripts. RBM8A is a ubiquitously expressed protein. Although RBM8A shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBM8A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for RBM8A Antibody (PCRP-RBM8A-1B4)

• Binder of OVCA1-1
• BOV-1
• BOV-1A
• BOV-1B
• BOV-1C
• MDS014
• RBM8BRBM8
• ribonucleoprotein RBM8
• Ribonucleoprotein RBM8A
• RNA binding motif protein 8A
• RNA binding motif protein 8B
• RNA-binding motif protein 8A
• RNA-binding protein 8A
• RNA-binding protein Y14
• Y14
• ZNRP
• ZRNP1

Background

Pre-mRNA splicing appears to play an important role in regulation of gene expression. It has been shown in a number of studies that during the splicing process, specific proteins are recruited to the mRNA and assemble into a complex of mRNA-protein near the exon-exon junction. This complex is termed mRNA-protein complex (mRNP), or the exon-exon junction complex. Several proteins were found to be present in this complex including Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK. Y14 is a 174 amino acid RNA-binding protein that contains one RNA-binding domain in the middle of the protein. Y14 binds to the spliced mRNAs in the nucleus and is exported with the mRNA to the cytoplasm. Similar to other proteins in the mRNA-protein complex, Y14 can interact with the mRNA export factor TAP and enhance the export of the mRNAs. Y14 binds to different mRNAs approx.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM8A
• Uniprot:
  • Q9Y5S9(Human)